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Literature summary for 6.3.1.8 extracted from

  • Pai, C.H.; Chiang, B.Y.; Ko, T.P.; Chou, C.C.; Chong, C.M.; Yen, F.J.; Chen, S.; Coward, J.K.; Wang, A.H.; Lin, C.H.
    Dual binding sites for translocation catalysis by Escherichia coli glutathionylspermidine synthetase (2006), EMBO J., 25, 5970-5982.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method generating crystals of GspS in 0.1M Tris–HCl (pH 8.5) containing 12% (v/v) PEG3350 and 0.5 M MgCl2 Escherichia coli

Protein Variants

Protein Variants Comment Organism
C338A increased Km compared to the wild type enzyme Escherichia coli
E391A increased Km compared to the wild type enzyme Escherichia coli
E392A increased Km compared to the wild type enzyme Escherichia coli
K607A increased Km compared to the wild type enzyme Escherichia coli
R316E no activity Escherichia coli
R538A increased Km compared to the wild type enzyme Escherichia coli
R598A decreased Km compared to the wild type enzyme Escherichia coli
S335A increased Km compared to the wild type enzyme Escherichia coli
S337A increased Km compared to the wild type enzyme Escherichia coli
T441A increased Km compared to the wild type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(2-[[([2-[(4-amino-4-carboxybutanoyl)amino]propanoyl]amino)methyl](hydroxy)phosphoryl]ethyl)(hydroxy)dioxophosphate(1-) inhibits the reaction in the presence of spermidine Escherichia coli
4-[(14-ammonio-5-hydroxy-1-methyl-5-oxido-2-oxo-6-oxa-3,10-diaza-5-phosphatetradec-1-yl)amino]-1-carboxy-4-oxobutan-1-aminium
-
 Escherichia coli
4-[[2-([[[4-[(4-ammoniobutyl)amino]butyl](hydroxy)phosphoryl]methyl]amino)-1-methyl-2-oxoethyl]amino]-1-carboxy-4-oxobutan-1-aminium
-
 Escherichia coli
gamma-glutamyl-N-[[(3-aminopropoxy)(2-phosphonoethyl)phosphoryl]methyl]alaninamide
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0224
-
 glutathione mutant enzyme R598A Escherichia coli
0.076
-
 spermidine wild type enzyme Escherichia coli
0.076
-
 spermidine mutant enzyme S337A Escherichia coli
0.126
-
 spermidine mutant enzyme S335A Escherichia coli
0.153
-
 spermidine mutant enzyme K607A Escherichia coli
0.182
-
 spermidine mutant enzyme R538A Escherichia coli
0.218
-
 glutathione wild type enzyme Escherichia coli
0.224
-
 glutathione mutant enzyme S337A Escherichia coli
0.251
-
 glutathione mutant enzyme K607A Escherichia coli
0.406
-
 spermidine mutant enzyme C338A Escherichia coli
0.407
-
 glutathione mutant enzyme E391A Escherichia coli
0.665
-
 spermidine mutant enzyme R598A Escherichia coli
0.691
-
 glutathione mutant enzyme T441A Escherichia coli
0.794
-
 glutathione mutant enzyme S335A Escherichia coli
1.008
-
 spermidine mutant enzyme E392A Escherichia coli
1.324
-
 glutathione mutant enzyme R538A Escherichia coli
1.61
-
 spermidine mutant enzyme T441A Escherichia coli
2.098
-
 glutathione mutant enzyme C338A Escherichia coli
4.522
-
 spermidine mutant enzyme E391A Escherichia coli
7.298
-
 glutathione mutant enzyme E392A Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ two Mg ions per molecule Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 2 * 70000, analytical ultracentrifugation Escherichia coli
138000
-
 analytical ultracentrifugation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AES0
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + spermidine + ATP
-
 Escherichia coli glutathionylspermidine + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 70000, analytical ultracentrifugation Escherichia coli

Synonyms

Synonyms Comment Organism
glutathionylspermidine synthetase
-
 Escherichia coli
GspS
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000078
-
 4-[[2-([[[4-[(4-ammoniobutyl)amino]butyl](hydroxy)phosphoryl]methyl]amino)-1-methyl-2-oxoethyl]amino]-1-carboxy-4-oxobutan-1-aminium
-
 Escherichia coli
0.006
-
 4-[(14-ammonio-5-hydroxy-1-methyl-5-oxido-2-oxo-6-oxa-3,10-diaza-5-phosphatetradec-1-yl)amino]-1-carboxy-4-oxobutan-1-aminium
-
 Escherichia coli