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Literature summary for 6.3.1.5 extracted from

  • McDonald, H.M.; Pruett, P.S.; Deivanayagam, C.; Protasevich, I.I.; Carson, W.M.; DeLucas, L.J.; Brouillette, W.J.; Brouillette, C.G.
    Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD+ synthetase from Bacillus anthracis (2007), Acta Crystallogr. Sect. D, 63, 891-905.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Bacillus anthracis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using 8-12% PEG 8000, 0.505 M ammonium sulfate, 6% glycerol, 100 mM magnesium chloride, 0.05% n-octyl-beta-D-glucopyranoside, 100 mM HEPES, pH 7 Bacillus anthracis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.152
-
deamido-NAD+ in 60 mM EPPS pH 8.5 with 20 mM ammonium chloride, 20 mM potassium chloride, and 10 mM magnesium chloride, at 37°C Bacillus anthracis
0.289
-
ATP in 60 mM EPPS pH 8.5 with 20 mM ammonium chloride, 20 mM potassium chloride, and 10 mM magnesium chloride, at 37°C Bacillus anthracis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus anthracis

Organism

Organism UniProt Comment Textmining
Bacillus anthracis Q81RP3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni HiTrap column chromatography, and Superdex-200 gel filtration or Superdex-75 gel filtration Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + NH3
-
Bacillus anthracis AMP + diphosphate + NAD+
-
?

Synonyms

Synonyms Comment Organism
NAD+ synthetase
-
Bacillus anthracis
NADS
-
Bacillus anthracis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bacillus anthracis

pH Range

pH Minimum pH Maximum Comment Organism
8.2 8.7
-
Bacillus anthracis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Bacillus anthracis