Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Bacillus anthracis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using 8-12% PEG 8000, 0.505 M ammonium sulfate, 6% glycerol, 100 mM magnesium chloride, 0.05% n-octyl-beta-D-glucopyranoside, 100 mM HEPES, pH 7 | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.152 | - |
deamido-NAD+ | in 60 mM EPPS pH 8.5 with 20 mM ammonium chloride, 20 mM potassium chloride, and 10 mM magnesium chloride, at 37°C | Bacillus anthracis | |
0.289 | - |
ATP | in 60 mM EPPS pH 8.5 with 20 mM ammonium chloride, 20 mM potassium chloride, and 10 mM magnesium chloride, at 37°C | Bacillus anthracis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | Q81RP3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni HiTrap column chromatography, and Superdex-200 gel filtration or Superdex-75 gel filtration | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + NH3 | - |
Bacillus anthracis | AMP + diphosphate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD+ synthetase | - |
Bacillus anthracis |
NADS | - |
Bacillus anthracis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus anthracis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.7 | - |
Bacillus anthracis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus anthracis |