Literature summary for 6.3.1.5 extracted from

Yamaguchi, F.; Koga, S.; Yoshioka, I.; Takahashi, M.; Sakuraba, H.; Ohshima, T.
Stable ammonia-specific NAD synthetase from Bacillus stearothermophilus: purification, characterization, gene cloning, and applications (2002), Biosci. Biotechnol. Biochem., 66, 2052-2059.

Search for more literature for 6.3.1.5

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, functional overexpression in Escherichia coli	Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.91	-	NH3	pH 8.5, 37°C	Geobacillus stearothermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required, optimal at 3 mM	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25000	-	2 * 25000, SDS-PAGE	Geobacillus stearothermophilus
50000	-	gel filtration	Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + deamido-NAD+ + NH3	Geobacillus stearothermophilus	-	AMP + diphosphate + NAD+	-	ir
ATP + deamido-NAD+ + NH3	Geobacillus stearothermophilus H-804	-	AMP + diphosphate + NAD+	-	ir

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	E07950	H-804	-
Geobacillus stearothermophilus H-804	E07950	H-804	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme to homogeneity	Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.59	-	purified native enzyme	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + deamido-NAD+ + NH3	-	Geobacillus stearothermophilus	AMP + diphosphate + NAD+	-	ir
ATP + deamido-NAD+ + NH3	specific for NH3, no activity with L-glutamine or other L-amino acids, no activity with urea, uric acid, or creatinine	Geobacillus stearothermophilus	AMP + diphosphate + NAD+	-	ir
ATP + deamido-NAD+ + NH3	-	Geobacillus stearothermophilus H-804	AMP + diphosphate + NAD+	-	ir
ATP + deamido-NAD+ + NH3	specific for NH3, no activity with L-glutamine or other L-amino acids, no activity with urea, uric acid, or creatinine	Geobacillus stearothermophilus H-804	AMP + diphosphate + NAD+	-	ir

Subunits

Subunits	Comment	Organism
dimer	2 * 25000, SDS-PAGE	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
ammonia-specific NAD synthetase	-	Geobacillus stearothermophilus
NAD synthase	-	Geobacillus stearothermophilus
NAD synthetase	-	Geobacillus stearothermophilus
Nicotinamide adenine dinucleotide synthetase	-	Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	stable at	Geobacillus stearothermophilus
70	-	40% of the activity at 60°C	Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	10	-	Geobacillus stearothermophilus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Geobacillus stearothermophilus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Geobacillus stearothermophilus	isoelectric focusing	-	5.3

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Release 2023.1 (January 2023)