Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ME8459 (glnA mutant strain) | Pyrococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
hydroxylamine | the Km value of GlnA for hydroxylamine is higher when a high concentration was used (5 to 30mM), 60°C, pH 7.8 | Pyrococcus sp. | |
23.5 | - |
L-glutamate | 60°C, pH 7.8 | Pyrococcus sp. | |
28 | - |
ATP | 60°C, pH 7.8 | Pyrococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
637000 | - |
gel filtration | Pyrococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus sp. | O08467 | - |
- |
Pyrococcus sp. KOD1 | O08467 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + hydroxylamine | - |
Pyrococcus sp. | ? | - |
? | |
ATP + L-glutamate + hydroxylamine | - |
Pyrococcus sp. KOD1 | ? | - |
? | |
additional information | in reactions in which Mn2+ is used, GTP, UTP, and CTP havea limited ability to replace ATP (less than 30%), while in reactions in which Mg2+ was used, complete inhibition by these NTPs is observed. Broad NTP specificity of hyperthermophilic archaeon synthetases suggests that enzymes of ancestral life forms can utilize various NTPs besides ATP | Pyrococcus sp. | ? | - |
? | |
additional information | in reactions in which Mn2+ is used, GTP, UTP, and CTP havea limited ability to replace ATP (less than 30%), while in reactions in which Mg2+ was used, complete inhibition by these NTPs is observed. Broad NTP specificity of hyperthermophilic archaeon synthetases suggests that enzymes of ancestral life forms can utilize various NTPs besides ATP | Pyrococcus sp. KOD1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | - |
Pyrococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
GlnA | - |
Pyrococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Pyrococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | 40°C: about 60% of maximal activity, 70°C: about 40% of maximal activity | Pyrococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life: 98 min | Pyrococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.83 | - |
hydroxylamine | 60°C, pH 7.8 | Pyrococcus sp. | |
36.5 | - |
L-glutamate | 60°C, pH 7.8 | Pyrococcus sp. | |
52.7 | - |
ATP | 60°C, pH 7.8 | Pyrococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Pyrococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.2 | pH 7.0: about 60% of maximal activity, pH 8.2: about 70% of maximal activity | Pyrococcus sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | hyperthermophilic archaea do not utilize glutamine synthetase predominantly for ammonia assimilation (the major pathway for ammonia assimilation is through glutamate dehydrogenase). The enzyme might play some role in ammonia assimilation under ammonia-starvation conditions | Pyrococcus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
hydroxylamine | the Km value of GlnA for hydroxylamine is higher when a high concentration was used (5 to 30mM), 60°C, pH 7.8 | Pyrococcus sp. | |
1.6 | - |
L-glutamate | 60°C, pH 7.8 | Pyrococcus sp. | |
1.9 | - |
ATP | 60°C, pH 7.8 | Pyrococcus sp. |