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Literature summary for 6.3.1.2 extracted from

  • Yadav, S.K.
    Computational structural analysis and kinetic studies of a cytosolic glutamine synthetase from Camellia sinensis (L.) O. Kuntze (2009), Protein J., 28, 428-434.
    View publication on PubMed
Search for more literature for 6.3.1.2

Crystallization (Commentary)

Crystallization (Comment) Organism
computational study on folding state Camellia sinensis

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive inhibitor with respect to ATP and noncompetitive inhibitor versus both glutamate and ammonium Camellia sinensis
L-glutamine noncompetitive Camellia sinensis
phosphate noncompetitive Camellia sinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2
-
 NH4+ pH 7.8 Camellia sinensis
6
-
 ATP pH 7.8 Camellia sinensis
9
-
 L-glutamate pH 7.8 Camellia sinensis

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Camellia sinensis A0MZ70
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine random catalysis mechanism where the binding order of certain substrates is kinetically preferred by the enzyme Camellia sinensis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + NH4+
-
 Camellia sinensis ADP + phosphate + L-glutamine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.6
-
 ATP pH 7.8 Camellia sinensis
13.5
-
 NH4+ pH 7.8 Camellia sinensis
15.2
-
 L-glutamate pH 7.8 Camellia sinensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 ADP substrate NH4+, pH 7.8 Camellia sinensis
0.47
-
 ADP substrate ATP, pH 7.8 Camellia sinensis
0.7
-
 ADP substrate L-glutamate, pH 7.8 Camellia sinensis
6.2
-
 phosphate substrate ATP, pH 7.8 Camellia sinensis
6.6
-
 L-glutamine substrate L-glutamate, pH 7.8 Camellia sinensis
7.4
-
 L-glutamine substrate NH4+, pH 7.8 Camellia sinensis
9.2
-
 L-glutamine substrate ATP, pH 7.8 Camellia sinensis
12.8
-
 phosphate substrate NH4+, pH 7.8 Camellia sinensis
17.3
-
 phosphate substrate L-glutamate, pH 7.8 Camellia sinensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.69
-
 L-glutamate pH 7.8 Camellia sinensis
1.9
-
 ATP pH 7.8 Camellia sinensis
2.6
-
 NH4+ pH 7.8 Camellia sinensis