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Literature summary for 6.3.1.2 extracted from
- Rapid determination of L-glutamine using engineered Escherichia coli overexpressing glutamine synthetase (2008), Appl. Biochem. Biotechnol., 158, 398-407.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional overexpression in Escherichia coli BL21 (DE3), expression analysis | Bacillus subtilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Bacillus subtilis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + NH3 | Bacillus subtilis | - |
ADP + phosphate + L-glutamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + hydroxylamine | in presence of Mn2+ | Bacillus subtilis | ADP + phosphate + gamma-L-glutamyl hydroxamate | - |
? | |
| ATP + L-glutamate + NH3 | - |
Bacillus subtilis | ADP + phosphate + L-glutamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glutamine synthetase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
assay at | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bacillus subtilis | |
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Release 2023.1 (January 2023)
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