Literature summary for 6.3.1.2 extracted from

Jaindl, M.; Popp, M.
Cyclitols protect glutamine synthetase and malate dehydrogenase against heat induced deactivation and thermal denaturation (2006), Biochem. Biophys. Res. Commun., 345, 761-765.

Activating Compound

Activating Compound	Comment	Organism
glycinebetaine	0.4 M, 67.7% residual activity after 60°C heat treatment	Escherichia coli
additional information	glucose shows little tendency to protect the enzyme from loss of activity	Escherichia coli
additional information	glucose shows little tendency to protect the enzyme from loss of activity	Hordeum vulgare
myo-inositol	0.4 M protects enzymatic activity during heat treatment	Hordeum vulgare
myo-inositol	0.4 M, 70.1% residual activity after 60°C heat treatment	Escherichia coli
pinitol	0.4 M results in maintenance of activity to 90% and 50% during heat treatment	Hordeum vulgare
pinitol	0.4 M, 74.2% residual activity after 60°C heat treatment	Escherichia coli
proline	0.4 M, 72.2% residual activity after 60°C heat treatment	Escherichia coli
quercitol	0.4 M, 75.5% residual activity after 60°C heat treatment	Escherichia coli
sorbitol	0.4 M, 62.0% residual activity after 60°C heat treatment	Escherichia coli

General Stability

General Stability	Organism
myo-inositol (0.4 M) protects enzymatic activity during heat treatment, pinitol (0.4 M) results in maintenance of activity to 90% and 50% during heat treatment, glucose shows little tendency to protect the enzyme from loss of activity	Hordeum vulgare
with myo-inositol (0.4 M) 70.1% residual activity after 60°C heat treatment, with pinitol (0.4 M) 74.2% residual activity after 60°C heat treatment, with quercitol (0.4 M) 75.5% residual activity after 60°C heat treatment, with sorbitol (0.4 M) 62.0% residual activity after 60°C heat treatment, with proline (0.4 M) 72.2% residual activity after 60°C heat treatment, with glycinebetain (0.4 M) 67.7% residual activity after 60°C heat treatment, glucose shows little tendency to protect the enzyme from loss of activity	Escherichia coli

Organism

myo-inositol (0.4 M) protects enzymatic activity during heat treatment, pinitol (0.4 M) results in maintenance of activity to 90% and 50% during heat treatment, glucose shows little tendency to protect the enzyme from loss of activity

Hordeum vulgare

with myo-inositol (0.4 M) 70.1% residual activity after 60°C heat treatment, with pinitol (0.4 M) 74.2% residual activity after 60°C heat treatment, with quercitol (0.4 M) 75.5% residual activity after 60°C heat treatment, with sorbitol (0.4 M) 62.0% residual activity after 60°C heat treatment, with proline (0.4 M) 72.2% residual activity after 60°C heat treatment, with glycinebetain (0.4 M) 67.7% residual activity after 60°C heat treatment, glucose shows little tendency to protect the enzyme from loss of activity

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Hordeum vulgare	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Makroprep HighQ chromatography	Hordeum vulgare

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Hordeum vulgare	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-Glu + NH4+	-	Escherichia coli	ADP + phosphate + L-Gln	-	?
ATP + L-Glu + NH4+	-	Hordeum vulgare	ADP + phosphate + L-Gln	-	?

Synonyms

Synonyms	Comment	Organism
L-Glutamine synthetase	-	Escherichia coli
L-Glutamine synthetase	-	Hordeum vulgare

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
35	-	retains normal activity after heating for 5 min at 35°C	Hordeum vulgare
40	55	activity decreases to 50% on heating at 40°C and to 10% or less on incubation at 55°C	Hordeum vulgare
60	80	deactivation in the temperature range 60-80°C	Escherichia coli