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Literature summary for 6.3.1.19 extracted from

  • Guth, E.; Thommen, M.; Weber-Ban, E.
    Mycobacterial ubiquitin-like protein ligase PafA follows a two-step reaction pathway with a phosphorylated pup intermediate (2011), J. Biol. Chem., 286, 4412-4419.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.19

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 [prokaryotic ubiquitin-like protein]-L-glutamate 25°C, pH 7.4 Mycobacterium tuberculosis
0.0087
-
 ATP 25°C, pH 7.4 Mycobacterium tuberculosis
0.0142
-
 [protein PanB]-L-lysine 25°C, pH 7.4 Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WNU7
-
-
Mycobacterium tuberculosis ATCC 25618 P9WNU7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein PanB]-L-lysine [protein PanB]-L-lysine shows a 3 orders of magnitude stronger affinity than free lysine Mycobacterium tuberculosis ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein PanB]-L-lysine
-
 ?
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein PanB]-L-lysine [protein PanB]-L-lysine shows a 3 orders of magnitude stronger affinity than free lysine Mycobacterium tuberculosis ATCC 25618 ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein PanB]-L-lysine
-
 ?
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine the enzyme catalyzes a two-step reaction mechanism proceeding through a gamma-glutamyl phosphate-mixed anhydride intermediate that is formed on the C-terminal glutamate of the prokaryotic ubiquitin-like protein (Pup) before transfer of Pup to the substrate acceptor lysine. Phosphorylated [prokaryotic ubiquitin-like protein]-L-glutamate and ADP are retained on the enzyme in a stable fashion following activation Mycobacterium tuberculosis ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine
-
 ?
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine the enzyme catalyzes a two-step reaction mechanism proceeding through a gamma-glutamyl phosphate-mixed anhydride intermediate that is formed on the C-terminal glutamate of the prokaryotic ubiquitin-like protein (Pup) before transfer of Pup to the substrate acceptor lysine. Phosphorylated [prokaryotic ubiquitin-like protein]-L-glutamate and ADP are retained on the enzyme in a stable fashion following activation Mycobacterium tuberculosis ATCC 25618 ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine
-
 ?

Synonyms

Synonyms Comment Organism
PafA ambiguous Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mycobacterium tuberculosis