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Literature summary for 6.3.1.19 extracted from

  • Forer, N.; Korman, M.; Elharar, Y.; Vishkautzan, M.; Gur, E.
    Bacterial proteasome and PafA, the pup ligase, interact to form a modular protein tagging and degradation machine (2013), Biochemistry, 52, 9029-9035.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
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Mycobacterium tuberculosis ATCC 25618
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Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme expressed in Escherichia coli Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
PafA ambiguous Mycobacterium tuberculosis
Pup ligase
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Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism proteasome-containing bacteria possess a tagging system that directs proteins to proteasomal degradation by conjugating them to a prokaryotic ubiquitin-like protein (Pup). A single ligating enzyme, PafA, is responsible for Pup conjugation to lysine side chains of protein substrates. As Pup is recognized by the regulatory subunit of the proteasome, Pup functions as a degradation tag. Ligating enzyme PafA and the proteasome can function as a modular machine for the tagging and degradation of cytoplasmic proteins Mycobacterium tuberculosis