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Literature summary for 6.3.1.17 extracted from

  • Collard, F.; Vertommen, D.; Constantinescu, S.; Buts, L.; Van Schaftingen, E.
    Molecular identification of beta-citrylglutamate hydrolase as glutamate carboxypeptidase 3 (2011), J. Biol. Chem., 286, 38220-38230.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.17

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
 beta-Citryl-L-glutamate in 25 mM HEPES, pH 7.1, at 30°C Mus musculus
0.0076
-
 N-acetyl-aspartylglutamate in 25 mM HEPES, pH 7.1, at 30°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ beta-citryl-L-glutamate is the preferred substrate in the presence of Ca2+ Mus musculus
Mg2+ Mg2+ stimulates very poorly the activity of the enzyme Mus musculus
Mn2+ The N-acetyl-aspartylglutamate hydrolase activity of the enzyme is stimulated by Mn2+, but not by Ca2+ Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphate + beta-citryl-L-glutamate Mus musculus isoform GCP3 acts preferentially on beta-citryl-L-glutamate in the presence of Ca2+. Its activity on this substrate is lower in the presence of Mn2+ and completely cancelled in the presence of Zn2+ ATP + citrate + L-glutamate
-
 ?
ADP + phosphate + N-acetyl-aspartylglutamate Mus musculus in the presence of Ca2+, the purified enzyme specifically hydrolyzes beta-citrylglutamate and does not act on N-acetyl-aspartylglutamate. However, both compounds were hydrolyzed in the presence of Mn2+ ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q80WS1
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, ConA-Sepharose column chromatography, and Superdex S-200 gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
bladder
-
 Mus musculus
-
kidney
-
 Mus musculus
-
lung
-
 Mus musculus
-
testis highest expression and activity Mus musculus
-
uterus
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphate + beta-citryl-L-glutamate isoform GCP3 acts preferentially on beta-citryl-L-glutamate in the presence of Ca2+. Its activity on this substrate is lower in the presence of Mn2+ and completely cancelled in the presence of Zn2+ Mus musculus ATP + citrate + L-glutamate
-
 ?
ADP + phosphate + N-acetyl-aspartylglutamate in the presence of Ca2+, the purified enzyme specifically hydrolyzes beta-citrylglutamate and does not act on N-acetyl-aspartylglutamate. However, both compounds were hydrolyzed in the presence of Mn2+ Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
GCP3
-
 Mus musculus
RIMKLB
-
 Mus musculus