Literature summary for 6.3.1.13 extracted from

Gutierrez-Lugo, M.T.; Newton, G.L.; Fahey, R.C.; Bewley, C.A.
Cloning, expression and rapid purification of active recombinant mycothiol ligase as B1 immunoglobulin binding domain of streptococcal protein G, glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis (2006), Protein Expr. Purif., 50, 128-136.

Application

Application	Comment	Organism
drug development	MshC may represent a novel target for new classes of antituberculars	Mycolicibacterium smegmatis

Cloned(Commentary)

Cloned (Comment)	Organism
gene mshC, construction of three N-terminal-MshC fusion proteins where N-terminal tags include the B1 domain of 1. streptococcal protein G to give GB1-MshC, 2. glutathione-S-transferase to give GST-MshC, and 3. maltose binding protein to give MBP-MshC, for expression in M. smegmatis, expression in enzyme-deficient mutant strain mc2155, i.e. I64 L205P, optimization of recombinant enzyme expression, overview	Mycolicibacterium smegmatis

Cloned (Comment)

Organism

gene mshC, construction of three N-terminal-MshC fusion proteins where N-terminal tags include the B1 domain of 1. streptococcal protein G to give GB1-MshC, 2. glutathione-S-transferase to give GST-MshC, and 3. maltose binding protein to give MBP-MshC, for expression in M. smegmatis, expression in enzyme-deficient mutant strain mc2155, i.e. I64 L205P, optimization of recombinant enzyme expression, overview

Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP	Mycolicibacterium smegmatis	key enzyme in the biosynthesis of mycothiol, pathway overview	1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged MshC and recombinant maltose binding protein-MshC by affinity chromatography on glutathione and amylose resins, respectively	Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Mycolicibacterium smegmatis
0.009	-	purified recombinant GST-tagged MshC	Mycolicibacterium smegmatis
0.025	-	purified recombinant maltose binding protein-MshC	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP	-	Mycolicibacterium smegmatis	1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate	-	?
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP	key enzyme in the biosynthesis of mycothiol, pathway overview	Mycolicibacterium smegmatis	1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
MshC	-	Mycolicibacterium smegmatis
mycothiol ligase	-	Mycolicibacterium smegmatis