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Literature summary for 6.3.1.13 extracted from
- A coupled spectrophotometric assay for l-cysteine:1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase and its application for inhibitor screening (2006), Anal. Biochem., 353, 167-173.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is a potential target for drugs directed against tuberculosis | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene mshC, expression in the mshC-deficient mutant strain I64 of Mycobacterium smegmatis, expression as insoluble protein in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | screening for inhibitor molecules | Mycobacterium tuberculosis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mycobacterium tuberculosis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycobacterium tuberculosis | the ATP-dependent ligation of Cys to GlcNIns is a key step in mycothiol biosynthesis, catalyzed by MshC, which is essential for growth of Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycobacterium tuberculosis Rv2130c | the ATP-dependent ligation of Cys to GlcNIns is a key step in mycothiol biosynthesis, catalyzed by MshC, which is essential for growth of Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
gene mshC | - |
| Mycobacterium tuberculosis Rv2130c | - |
gene mshC | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from mshC-deficient mutant strain I64 of Mycobacterium smegmatis by ammonium sulfate fractionation, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
spectrophotometric assay method development with coupling of diphosphatase to convert diphosphate to phosphate and spectrophotometric detection of the latter via the phosphomolybdate complex with malachite green, overview | Mycobacterium tuberculosis |
| 0.15 | - |
purified recombinant enzyme | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | the ATP-dependent ligation of Cys to GlcNIns is a key step in mycothiol biosynthesis, catalyzed by MshC, which is essential for growth of Mycobacterium tuberculosis | Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycobacterium tuberculosis Rv2130c | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | the ATP-dependent ligation of Cys to GlcNIns is a key step in mycothiol biosynthesis, catalyzed by MshC, which is essential for growth of Mycobacterium tuberculosis | Mycobacterium tuberculosis Rv2130c | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-cysteine:1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase | - |
Mycobacterium tuberculosis |
| MshC | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mycobacterium tuberculosis | |
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Release 2023.1 (January 2023)
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