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Literature summary for 6.3.1.12 extracted from

  • Staudenbauer, W.; Strominger, J.L.
    Biosynthesis of the peptidoglycan of bacterial cell walls. XXII. Activation of D-aspartic acid for incorporation into peptidoglycan (1972), J. Biol. Chem., 247, 5095-5102.
    View publication on PubMed
Search for more literature for 6.3.1.12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 ATP
-
 Enterococcus faecalis
2
-
 D-Aspartate
-
 Enterococcus faecalis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Enterococcus faecalis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ a divalent cation is required for activity. Mg2+ and Mn2+ are approximately equally effective. Optimal concentration of Mg2+ is 50 mM or greater Enterococcus faecalis
Mn2+ a divalent cation is required for activity. Mg2+ and Mn2+ are approximately equally effective. Optimal concentration of Mg2+ is 20 mM Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-
Lacticaseibacillus casei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterococcus faecalis
-
 Lacticaseibacillus casei

Storage Stability

Storage Stability Organism
-15°C, precipitation with ammonium sulfate, stable for at least several weeks Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-aspartate + [beta-GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n strictly specific for ATP. No activity with CTP, GTP, TTP and UTP Enterococcus faecalis [beta-GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala)]n + ADP + phosphate
-
 ?
ATP + D-aspartate + [beta-GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n strictly specific for ATP. No activity with CTP, GTP, TTP and UTP Lacticaseibacillus casei [beta-GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala)]n + ADP + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Enterococcus faecalis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: about 40% of maximal activity, pH 8.5: about 70% of maximal activity Enterococcus faecalis