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Literature summary for 6.2.1.B3 extracted from

  • Kienow, L.; Schneider, K.; Bartsch, M.; Stuible, H.P.; Weng, H.; Miersch, O.; Wasternack, C.; Kombrink, E.
    Jasmonates meet fatty acids: functional analysis of a new acyl-coenzyme A synthetase family from Arabidopsis thaliana (2008), J. Exp. Bot., 59, 403-419.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.027
-
12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.036
-
tetradecanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.076
-
dinor-12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.094
-
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Arabidopsis thaliana 5777
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q84P21
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12-oxo-phytodienoic acid + CoA + ATP
-
Arabidopsis thaliana 12-oxo-phytodienoyl-CoA + AMP + diphosphate
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA + ATP i.e. OPC-6:0 Arabidopsis thaliana 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA + AMP + diphosphate
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid + CoA + ATP i.e. OPC-8:0 Arabidopsis thaliana 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
-
?
decanoic acid + CoA + ATP
-
Arabidopsis thaliana decanoyl-CoA + AMP + diphosphate
-
?
dinor-12-oxo-phytodienoic acid + CoA + ATP
-
Arabidopsis thaliana dinor-12-oxo-phytodienoyl-CoA + AMP + diphosphate
-
?
dodecanoic acid + CoA + ATP
-
Arabidopsis thaliana dodecanoyl-CoA + AMP + diphosphate
-
?
hexadecanoic acid + CoA + ATP
-
Arabidopsis thaliana hexadecanoyl-CoA + AMP + diphosphate
-
?
octadecanoic acid + CoA + ATP
-
Arabidopsis thaliana octadecanoyl-CoA + AMP + diphosphate
-
?
oleic acid + CoA + ATP
-
Arabidopsis thaliana oleoyl-CoA + AMP + diphosphate
-
?
tetradecanoic acid + CoA + ATP
-
Arabidopsis thaliana tetradecanoyl-CoA + AMP + diphosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.96
-
tetradecanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
1.27
-
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
1.49
-
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
1.56
-
12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
1.78
-
dinor-12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana expression is activated in response to wounding or infection up

General Information

General Information Comment Organism
physiological function mutants exhibit 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid accumulation and reduced levels of jasmonic acid. Enzyme catalyzes an essential step in jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of the carboxylic acid side chain of its precursors Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.2
-
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
23.4
-
dinor-12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
26.4
-
tetradecanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
56.2
-
12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
65.4
-
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana