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Literature summary for 6.2.1.B1 extracted from
- o-Succinylbenzoate:coenzyme A ligase from anthraquinone producing cell suspension cultures of Galium mollugo (1992), Phytochemistry, 31, 2329-2335.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | less efficient activation than Mg2+ | Galium mollugo |  |
| Mg2+ | activates | Galium mollugo | |
| Mn2+ | less efficient activation than Mg2+ | Galium mollugo | |
| Ni2+ | less efficient activation than Mg2+ | Galium mollugo | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DIECA | - |
Galium mollugo | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0599 | - |
CoA | - |
Galium mollugo | |
| 0.0881 | - |
ATP | - |
Galium mollugo | |
| 0.1158 | - |
o-succinylbenzoate | - |
Galium mollugo | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-succinylbenzoate + CoA | Galium mollugo | the enzyme is involved in anthraquinone biosynthesis | ADP + phosphate + 2-succinylbenzoyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Galium mollugo | - |
- |
- |
| Galium verum | - |
- |
- |
| Rubia tinctorum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Galium mollugo |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Galium mollugo | - |
| cell culture | - |
Rubia tinctorum | - |
| cell culture | - |
Galium verum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000834 | - |
- |
Galium mollugo |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, enzyme in crude extract has a half-life of 672 h | Galium mollugo |
| 4°C, enzyme in crude extract has a half-life of 37 h | Galium mollugo |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-succinylbenzoate + CoA | - |
Galium mollugo | ADP + phosphate + 2-succinylbenzoyl-CoA | - |
? | |
| ATP + 2-succinylbenzoate + CoA | the enzyme is involved in anthraquinone biosynthesis | Galium mollugo | ADP + phosphate + 2-succinylbenzoyl-CoA | - |
? | |
| ATP + 2-succinylbenzoate + dephospho-CoA | 26% of the activity with CoA | Galium mollugo | ADP + phosphate + 2-succinylbenzoyl-CoA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
- |
Galium mollugo |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
enzyme in crude extract, half-life: 9.3 h | Galium mollugo |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Galium mollugo |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Galium mollugo | phosphoralyted enzyme form, isoelectric focusing | - |
7.5 |
| Galium mollugo | non-phosphorylated enzyme form, isoelectric focusing | - |
8.7 |
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