Cloned (Comment) | Organism |
---|---|
gene sucCD, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)/pLysS | Advenella mimigardefordensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | transposon mutagenesis and deletion of gene sucCD, accumulation of 3SP during 3,3'-dithiodipropionic acid degradation occurs in Tn5::mob-induced mutants of Advenella mimigardefordensis strain DPN7T disrupted in sucCD and in the defined deletion mutant Advenella mimigardefordensis DELTAsucCD | Advenella mimigardefordensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
CoA | pH 7.4, 30°C, recombinant sucCD | Advenella mimigardefordensis | |
0.083 | - |
ATP | pH 7.4, 30°C, recombinant sucCD | Advenella mimigardefordensis | |
0.143 | - |
succinate | pH 7.4, 30°C, recombinant sucCD | Advenella mimigardefordensis | |
0.448 | - |
Itaconate | pH 7.4, 30°C, recombinant sucCD | Advenella mimigardefordensis | |
0.818 | - |
Itaconate | pH 7.4, 30°C, recombinant sucCD | Advenella mimigardefordensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | SucCDAm is Mg2+- or Mn2+-dependent | Advenella mimigardefordensis | |
Mn2+ | SucCDAm is Mg2+- or Mn2+-dependent | Advenella mimigardefordensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + succinate + CoA | Advenella mimigardefordensis | SucCDAm is unspecific regarding ATP or GTP | ADP + phosphate + succinyl-CoA | - |
? | |
ATP + succinate + CoA | Advenella mimigardefordensis DPN7T | SucCDAm is unspecific regarding ATP or GTP | ADP + phosphate + succinyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Advenella mimigardefordensis | B3TZD8 | subunit beta; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm | - |
Advenella mimigardefordensis | B3TZD9 | subunit alpha; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm | - |
Advenella mimigardefordensis DPN7T | B3TZD8 | subunit beta; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm | - |
Advenella mimigardefordensis DPN7T | B3TZD9 | subunit alpha; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble enzyme from Escherichia coli strain BL21(DE3)/pLysS by anion exchange chromatography | Advenella mimigardefordensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-sulfinopropionate + CoA | SucCDAm is unspecific regarding ATP or GTP | Advenella mimigardefordensis | ADP + phosphate + 3-sulfinopropionyl-CoA | determination of 3-sulfinopropionyl-CoA structure by using liquid chromatography-electrospray ionization-mass spectrometry | ? | |
ATP + itaconate + CoA | SucCDAm is unspecific regarding ATP or GTP | Advenella mimigardefordensis | ADP + phosphate + itaconyl-CoA | - |
? | |
ATP + succinate + CoA | SucCDAm is unspecific regarding ATP or GTP | Advenella mimigardefordensis | ADP + phosphate + succinyl-CoA | - |
? | |
ATP + succinate + CoA | succinate is the best substrate, SucCDAm is unspecific regarding ATP or GTP | Advenella mimigardefordensis | ADP + phosphate + succinyl-CoA | - |
? | |
ATP + succinate + CoA | SucCDAm is unspecific regarding ATP or GTP | Advenella mimigardefordensis DPN7T | ADP + phosphate + succinyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SucCDAm | - |
Advenella mimigardefordensis |
Succinyl coenzyme A synthetase | - |
Advenella mimigardefordensis |
Succinyl-CoA synthetase | - |
Advenella mimigardefordensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Advenella mimigardefordensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Advenella mimigardefordensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | SucCDAm is unspecific regarding ATP or GTP | Advenella mimigardefordensis |