Cloned (Comment) | Organism |
---|---|
gene CrUBE1, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons, semi-quantitative RT-PCR and quantitative real-time PCR enzyme expression anayses, recombinant expression in Pichia pastoris strain GS115 | Citrus reticulata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Citrus reticulata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49280 | - |
x * 49280, sequence calculation | Citrus reticulata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine | Citrus reticulata | - |
AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus reticulata | - |
cv. Wuzishatangju (seedless) and cv. Shatangju (seedy), single copy gene CrUBE1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
anther | expression level of the CrUBE1 gene in anthers of cv. Shatangju is approximately 10fold higher than in anthers of cv. Wuzishatangju | Citrus reticulata | - |
carpel | high enzyme expression level | Citrus reticulata | - |
filament | very low expression | Citrus reticulata | - |
flower bud | - |
Citrus reticulata | - |
leaf | very low expression | Citrus reticulata | - |
leaf bud | - |
Citrus reticulata | - |
additional information | semi-quantitative and quantitative expressio analysis of the enzyme in different tissues of the two cultivars, o verview, the highest expression level of CrUBE1 is detected in pistils at 7 days after self pollination of cv.Wuzishatangju, which is approximately 5fold higher than at the starting point | Citrus reticulata | - |
petal | very low expression | Citrus reticulata | - |
plant ovary | - |
Citrus reticulata | - |
stigma | - |
Citrus reticulata | - |
style | very low expression | Citrus reticulata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine | - |
Citrus reticulata | AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49280, sequence calculation | Citrus reticulata |
Synonyms | Comment | Organism |
---|---|---|
UBE1 | - |
Citrus reticulata |
ubiquitin-activating enzyme E1 | - |
Citrus reticulata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Citrus reticulata |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Citrus reticulata | sequence calculation | - |
5.44 |
Organism | Comment | Expression |
---|---|---|
Citrus reticulata | self pollination induces the enzyme in cv. Wuzishatangju | up |
General Information | Comment | Organism |
---|---|---|
evolution | 5 amino acids and 8 bases are different in cDNA and DNA sequences of CrUBE1 between Wuzishatangju and Shatangju, respectively | Citrus reticulata |
metabolism | ubiquitin-activating enzyme E1 catalyzes the first step in the ubiquitination reaction, which targets a protein for degradation via a proteasome pathway. The enzyme plays an important role in metabolic processes | Citrus reticulata |
additional information | the enzyme has six ATP binding sites, one catalytic residue, and one E2 binding domain | Citrus reticulata |
physiological function | UBE1s catalyze the first steps in the ubiquitin conjugation cascade and are potentially involved in compatible pollination and ubiquitinmediated S-RNase degradation | Citrus reticulata |