Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | UBE1L2 transfers activated ubiquitin onto UbcH5b and supports E3-mediated polyubiquitylation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | A0AVT1 | - |
- |
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
SW-60 cell | colon carcinoma cell | Homo sapiens | - |
testis | the UBE1L2 mRNA is most abundantly expressed in the testis | Mus musculus | - |
testis | the UBE1L2 mRNA is most abundantly expressed in the testis | Homo sapiens | - |
testis | most abundant expression | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ubiquitin + [ubiquitin-activating protein Uba6]-L-cysteine | isoform Uba6 forms a covalent link with ubiquitin in vitro and in vivo, which is sensitive to reducing conditions. Recombinant E1 enzyme Uba6 can activate ubiquitin and transfer it onto the ubiquitin-conjugating enzyme UbcH5B. Ubiquitin activated by Uba6 can be used for ubiquitylation of p53 and supports the autoubiquitylation of the E3 ubiquitin ligases HectH9 and E6-AP | Homo sapiens | AMP + diphosphate + [ubiquitin-activating protein Uba6]-S-ubiquitinyl-L-cysteine | - |
? | |
additional information | UBE1L2 transfers activated ubiquitin onto UbcH5b and supports E3-mediated polyubiquitylation | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Uba6 | - |
Homo sapiens |
UBE1L2 | - |
Mus musculus |
UBE1L2 | - |
Homo sapiens |
ubiquitin-activating enzyme 6 | - |
Homo sapiens |
ubiquitin-activating enzyme E1 | - |
Mus musculus |
ubiquitin-activating enzyme E1 | - |
Homo sapiens |
ubiquitin-like modifier-activating enzyme 6 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | isoform Uba6 forms a covalent link with ubiquitin in vitro and in vivo, which is sensitive to reducing conditions. In an in vitro polyubiquitylation assay, recombinant Uba6 can activate ubiquitin and transfer it onto the ubiquitin-conjugating enzyme UbcH5B. Ubiquitin activated by Uba6 can be used for ubiquitylation of p53 by MDM2 and supports the autoubiquitylation of the E3 ubiquitin ligases HectH9 and E6-AP | Homo sapiens |