Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.2.1.36 extracted from

  • Alber, B.E.; Kung, J.W.; Fuchs, G.
    3-Hydroxypropionyl-coenzyme A synthetase from Metallosphaera sedula, an enzyme involved in autotrophic CO2 fixation (2008), J. Bacteriol., 190, 1383-1389.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
ATP pH 8.5, 45°C Metallosphaera sedula
0.12
-
propionate pH 8.5, 45°C Metallosphaera sedula
0.18
-
3-hydroxypropionate pH 8.5, 45°C Metallosphaera sedula
1.42
-
Acrylate pH 8.5, 45°C Metallosphaera sedula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
2 * 74000, SDS-PAGE Sulfurisphaera tokodaii
78000
-
4 * 78000, SDS-PAGE Metallosphaera sedula
140000
-
gel filtration Sulfurisphaera tokodaii
340000
-
gel filtration Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxypropanoate + ATP + coenzyme A Metallosphaera sedula the enzyme is involved in autotrophic CO2 fixation 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
?
3-hydroxypropanoate + ATP + coenzyme A Sulfurisphaera tokodaii the enzyme is involved in autotrophic CO2 fixation 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
?
3-hydroxypropanoate + ATP + coenzyme A Sulfurisphaera tokodaii DSM 16993 the enzyme is involved in autotrophic CO2 fixation 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
?
3-hydroxypropanoate + ATP + coenzyme A Metallosphaera sedula DSM 5348 the enzyme is involved in autotrophic CO2 fixation 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YGR1
-
-
Metallosphaera sedula DSM 5348 A4YGR1
-
-
Sulfurisphaera tokodaii Q973W5
-
-
Sulfurisphaera tokodaii DSM 16993 Q973W5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Metallosphaera sedula
recombinant enzyme Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.7
-
pH 8,5, 65°C Sulfurisphaera tokodaii
18
-
pH 8,5, 45°C, autotrophically grown cells Metallosphaera sedula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloropropionate + ATP + coenzyme A 64% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii 3-chloropropionyl-CoA + AMP + diphosphate
-
?
3-chloropropionate + ATP + coenzyme A 64% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii DSM 16993 3-chloropropionyl-CoA + AMP + diphosphate
-
?
3-hydroxypropanoate + ATP + coenzyme A
-
Sulfurisphaera tokodaii 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
?
3-hydroxypropanoate + ATP + coenzyme A the enzyme is involved in autotrophic CO2 fixation Metallosphaera sedula 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
?
3-hydroxypropanoate + ATP + coenzyme A the enzyme is involved in autotrophic CO2 fixation Sulfurisphaera tokodaii 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
?
3-hydroxypropanoate + ATP + coenzyme A the enzyme is specific for 3-hydroxypropionate and propionate Metallosphaera sedula 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
?
3-hydroxypropanoate + ATP + coenzyme A
-
Sulfurisphaera tokodaii DSM 16993 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
?
3-hydroxypropanoate + ATP + coenzyme A the enzyme is involved in autotrophic CO2 fixation Sulfurisphaera tokodaii DSM 16993 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
?
3-hydroxypropanoate + ATP + coenzyme A the enzyme is involved in autotrophic CO2 fixation Metallosphaera sedula DSM 5348 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
?
3-hydroxypropanoate + CTP + coenzyme A 13% of the activity with ATP Metallosphaera sedula 3-hydroxypropanoyl-CoA + CMP + diphosphate
-
?
3-hydroxypropanoate + UTP + coenzyme A 32% of the activity with ATP Metallosphaera sedula 3-hydroxypropanoyl-CoA + UMP + diphosphate
-
?
3-mercaptopropionate + ATP + coenzyme A 36% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii 3-mercaptopropionyl-CoA + AMP + diphosphate
-
?
3-mercaptopropionate + ATP + coenzyme A 36% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii DSM 16993 3-mercaptopropionyl-CoA + AMP + diphosphate
-
?
acetate + ATP + coenzyme A 42% of the activity with 3-hydroxypropionate Metallosphaera sedula acetyl-CoA + AMP + diphosphate
-
?
acetate + ATP + coenzyme A 65% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii acetyl-CoA + AMP + diphosphate
-
?
acrylate + ATP + coenzyme A 77% of the activity with 3-hydroxypropionate Metallosphaera sedula acryloyl-CoA + AMP + diphosphate
-
?
acrylate + ATP + coenzyme A 96% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii acryloyl-CoA + AMP + diphosphate
-
?
butyrate + ATP + coenzyme A 20% of the activity with 3-hydroxypropionate Metallosphaera sedula butyryl-CoA + AMP + diphosphate
-
?
butyrate + ATP + coenzyme A 27% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii butyryl-CoA + AMP + diphosphate
-
?
additional information less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate Metallosphaera sedula ?
-
?
additional information less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate Sulfurisphaera tokodaii ?
-
?
additional information less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate Sulfurisphaera tokodaii DSM 16993 ?
-
?
additional information less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate Metallosphaera sedula DSM 5348 ?
-
?
propionate + ATP + coenzyme A 98% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii propionyl-CoA + AMP + diphosphate
-
?
propionate + ATP + coenzyme A the enzyme is specific for 3-hydroxypropionate and propionate Metallosphaera sedula propionyl-CoA + AMP + diphosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 74000, SDS-PAGE Sulfurisphaera tokodaii
homotetramer 4 * 78000, SDS-PAGE Metallosphaera sedula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
assay at Metallosphaera sedula
65
-
assay at Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
15 min, stable up to 80°C Metallosphaera sedula
100
-
15 min, 50% loss of activity Metallosphaera sedula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4
-
Acrylate pH 8.5, 45°C Metallosphaera sedula
5.6
-
propionate pH 8.5, 45°C Metallosphaera sedula
5.7
-
3-hydroxypropionate pH 8.5, 45°C Metallosphaera sedula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Metallosphaera sedula
8.5
-
assay at Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.8
-
Acrylate pH 8.5, 45°C Metallosphaera sedula
31.7
-
3-hydroxypropionate pH 8.5, 45°C Metallosphaera sedula
46.7
-
propionate pH 8.5, 45°C Metallosphaera sedula