Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
ATP | pH 8.5, 45°C | Metallosphaera sedula | |
0.12 | - |
propionate | pH 8.5, 45°C | Metallosphaera sedula | |
0.18 | - |
3-hydroxypropionate | pH 8.5, 45°C | Metallosphaera sedula | |
1.42 | - |
Acrylate | pH 8.5, 45°C | Metallosphaera sedula |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
2 * 74000, SDS-PAGE | Sulfurisphaera tokodaii |
78000 | - |
4 * 78000, SDS-PAGE | Metallosphaera sedula |
140000 | - |
gel filtration | Sulfurisphaera tokodaii |
340000 | - |
gel filtration | Metallosphaera sedula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxypropanoate + ATP + coenzyme A | Metallosphaera sedula | the enzyme is involved in autotrophic CO2 fixation | 3-hydroxypropanoyl-CoA + AMP + diphosphate | - |
? | |
3-hydroxypropanoate + ATP + coenzyme A | Sulfurisphaera tokodaii | the enzyme is involved in autotrophic CO2 fixation | 3-hydroxypropanoyl-CoA + AMP + diphosphate | - |
? | |
3-hydroxypropanoate + ATP + coenzyme A | Sulfurisphaera tokodaii DSM 16993 | the enzyme is involved in autotrophic CO2 fixation | 3-hydroxypropanoyl-CoA + AMP + diphosphate | - |
? | |
3-hydroxypropanoate + ATP + coenzyme A | Metallosphaera sedula DSM 5348 | the enzyme is involved in autotrophic CO2 fixation | 3-hydroxypropanoyl-CoA + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | A4YGR1 | - |
- |
Metallosphaera sedula DSM 5348 | A4YGR1 | - |
- |
Sulfurisphaera tokodaii | Q973W5 | - |
- |
Sulfurisphaera tokodaii DSM 16993 | Q973W5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Metallosphaera sedula |
recombinant enzyme | Sulfurisphaera tokodaii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.7 | - |
pH 8,5, 65°C | Sulfurisphaera tokodaii |
18 | - |
pH 8,5, 45°C, autotrophically grown cells | Metallosphaera sedula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chloropropionate + ATP + coenzyme A | 64% of the activity with 3-hydroxypropionate | Sulfurisphaera tokodaii | 3-chloropropionyl-CoA + AMP + diphosphate | - |
? | |
3-chloropropionate + ATP + coenzyme A | 64% of the activity with 3-hydroxypropionate | Sulfurisphaera tokodaii DSM 16993 | 3-chloropropionyl-CoA + AMP + diphosphate | - |
? | |
3-hydroxypropanoate + ATP + coenzyme A | - |
Sulfurisphaera tokodaii | 3-hydroxypropanoyl-CoA + AMP + diphosphate | - |
? | |
3-hydroxypropanoate + ATP + coenzyme A | the enzyme is involved in autotrophic CO2 fixation | Metallosphaera sedula | 3-hydroxypropanoyl-CoA + AMP + diphosphate | - |
? | |
3-hydroxypropanoate + ATP + coenzyme A | the enzyme is involved in autotrophic CO2 fixation | Sulfurisphaera tokodaii | 3-hydroxypropanoyl-CoA + AMP + diphosphate | - |
? | |
3-hydroxypropanoate + ATP + coenzyme A | the enzyme is specific for 3-hydroxypropionate and propionate | Metallosphaera sedula | 3-hydroxypropanoyl-CoA + AMP + diphosphate | - |
? | |
3-hydroxypropanoate + ATP + coenzyme A | - |
Sulfurisphaera tokodaii DSM 16993 | 3-hydroxypropanoyl-CoA + AMP + diphosphate | - |
? | |
3-hydroxypropanoate + ATP + coenzyme A | the enzyme is involved in autotrophic CO2 fixation | Sulfurisphaera tokodaii DSM 16993 | 3-hydroxypropanoyl-CoA + AMP + diphosphate | - |
? | |
3-hydroxypropanoate + ATP + coenzyme A | the enzyme is involved in autotrophic CO2 fixation | Metallosphaera sedula DSM 5348 | 3-hydroxypropanoyl-CoA + AMP + diphosphate | - |
? | |
3-hydroxypropanoate + CTP + coenzyme A | 13% of the activity with ATP | Metallosphaera sedula | 3-hydroxypropanoyl-CoA + CMP + diphosphate | - |
? | |
3-hydroxypropanoate + UTP + coenzyme A | 32% of the activity with ATP | Metallosphaera sedula | 3-hydroxypropanoyl-CoA + UMP + diphosphate | - |
? | |
3-mercaptopropionate + ATP + coenzyme A | 36% of the activity with 3-hydroxypropionate | Sulfurisphaera tokodaii | 3-mercaptopropionyl-CoA + AMP + diphosphate | - |
? | |
3-mercaptopropionate + ATP + coenzyme A | 36% of the activity with 3-hydroxypropionate | Sulfurisphaera tokodaii DSM 16993 | 3-mercaptopropionyl-CoA + AMP + diphosphate | - |
? | |
acetate + ATP + coenzyme A | 42% of the activity with 3-hydroxypropionate | Metallosphaera sedula | acetyl-CoA + AMP + diphosphate | - |
? | |
acetate + ATP + coenzyme A | 65% of the activity with 3-hydroxypropionate | Sulfurisphaera tokodaii | acetyl-CoA + AMP + diphosphate | - |
? | |
acrylate + ATP + coenzyme A | 77% of the activity with 3-hydroxypropionate | Metallosphaera sedula | acryloyl-CoA + AMP + diphosphate | - |
? | |
acrylate + ATP + coenzyme A | 96% of the activity with 3-hydroxypropionate | Sulfurisphaera tokodaii | acryloyl-CoA + AMP + diphosphate | - |
? | |
butyrate + ATP + coenzyme A | 20% of the activity with 3-hydroxypropionate | Metallosphaera sedula | butyryl-CoA + AMP + diphosphate | - |
? | |
butyrate + ATP + coenzyme A | 27% of the activity with 3-hydroxypropionate | Sulfurisphaera tokodaii | butyryl-CoA + AMP + diphosphate | - |
? | |
additional information | less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate | Metallosphaera sedula | ? | - |
? | |
additional information | less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate | Sulfurisphaera tokodaii | ? | - |
? | |
additional information | less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate | Sulfurisphaera tokodaii DSM 16993 | ? | - |
? | |
additional information | less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate | Metallosphaera sedula DSM 5348 | ? | - |
? | |
propionate + ATP + coenzyme A | 98% of the activity with 3-hydroxypropionate | Sulfurisphaera tokodaii | propionyl-CoA + AMP + diphosphate | - |
? | |
propionate + ATP + coenzyme A | the enzyme is specific for 3-hydroxypropionate and propionate | Metallosphaera sedula | propionyl-CoA + AMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 74000, SDS-PAGE | Sulfurisphaera tokodaii |
homotetramer | 4 * 78000, SDS-PAGE | Metallosphaera sedula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Metallosphaera sedula |
65 | - |
assay at | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
15 min, stable up to 80°C | Metallosphaera sedula |
100 | - |
15 min, 50% loss of activity | Metallosphaera sedula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
Acrylate | pH 8.5, 45°C | Metallosphaera sedula | |
5.6 | - |
propionate | pH 8.5, 45°C | Metallosphaera sedula | |
5.7 | - |
3-hydroxypropionate | pH 8.5, 45°C | Metallosphaera sedula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Metallosphaera sedula |
8.5 | - |
assay at | Sulfurisphaera tokodaii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
Acrylate | pH 8.5, 45°C | Metallosphaera sedula | |
31.7 | - |
3-hydroxypropionate | pH 8.5, 45°C | Metallosphaera sedula | |
46.7 | - |
propionate | pH 8.5, 45°C | Metallosphaera sedula |