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Literature summary for 6.2.1.33 extracted from

  • Loeffler, F.; Mueller, R.; Lingens, F.
    Purification and properties of 4-halobenzoate-coenzyme A ligase from Pseudomonas sp. CBS3 (1992), Biol. Chem. Hoppe-Seyler, 373, 1001-1007.
    View publication on PubMed
Search for more literature for 6.2.1.33

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Pseudomonas sp.
Fe2+
-
 Pseudomonas sp.
SDS 1 mM, irreversibe Pseudomonas sp.
Zn2+
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05 0.065 4-Chlorobenzoate
-
 Pseudomonas sp.
0.11 0.19 CoA
-
 Pseudomonas sp.
2.4 3.5 ATP
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 77% of the activation with Mg2+ Pseudomonas sp.
Fe2+ 70% of the activation with Mg2+ Pseudomonas sp.
Mg2+ required Pseudomonas sp.
Mg2+ maximal initial rate of catalysis is achieved with an Mg2+:ATP ratio of 1:0.66 to 1:1.33 Pseudomonas sp.
Mn2+ 87% of the activation with Mg2+ Pseudomonas sp.
Ni2+ 11% of the activation with Mg2+ Pseudomonas sp.
Zn2+ 23% of the activation with Mg2+ Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 2 * 57000, SDS-PAGE Pseudomonas sp.
86000 120000 gel filtration Pseudomonas sp.
125000
-
 native gradient PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
111.6
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, stable for months Pseudomonas sp.
4°C, 0.1-2.5 mg of protein per ml, half-life: 21 days Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Bromobenzoate + CoA + ATP at 105% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Bromobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Chlorobenzoate + CoA + ADP reaction with ADP at 7.5% of the reaction with ATP Pseudomonas sp. 4-Chlorobenzoyl-CoA + AMP + phosphate
-
 ?
4-Chlorobenzoate + CoA + ATP negligible activity with UTP, CTP, and GTP Pseudomonas sp. 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Fluorobenzoate + CoA + ATP at 36% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Fluorobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Iodobenzoate + CoA + ATP at 90% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Iodobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Methoxybenzoate + CoA + ATP at 9% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Methoxybenzoyl-CoA + AMP + diphosphate
-
 ?
4-Methylbenzoate + CoA + ATP at 22% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Methylbenzoyl-CoA + AMP + diphosphate
-
 ?
4-Nitrobenzoate + CoA + ATP at 7% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Nitrobenzoyl-CoA + AMP + diphosphate
-
 ?
Benzoate + CoA + ATP at 5% of the activity with 4-chlorobenzoate Pseudomonas sp. Benzoyl-CoA + AMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 57000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 0.1-2.5 mg of protein per ml, half-life: 21 days Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
 Pseudomonas sp.