Crystallization (Comment) | Organism |
---|---|
purified recombinant CBL bound to 4-chlorobenzoyl-AMP, hanging drop vapor diffusion at 4°C using 16-24% pentaerythritol propoxylate 426, and 0.1 M K+-HEPES, pH 6.5 or 6.75, and optimized via hanging drop vapor diffusion using 14-18% PEG 1000, 50-100 mM magnesium nitrate and 100 mM MOPS, pH 7.0, the cryoprotectant contains 24% pentaerythritol propoxylate 426, 24% ethylene glycol, and 0.1 M K+-HEPES, 1 mM ATP, and 1 mM 4-chlorobenzoate, X-ray diffraction structure determination and analysis at 2.0-2.25 A resolution, molecular replacement | Alcaligenes sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chlorophenacyl-CoA | the product analogue traps the enzyme in the thioester-forming conformation, binding structure, overview | Alcaligenes sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Alcaligenes sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Chlorobenzoate + CoA + ATP | Alcaligenes sp. | - |
4-Chlorobenzoyl-CoA + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes sp. | Q8GN86 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-Chlorobenzoate + CoA + ATP = 4-chlorobenzoyl-CoA + AMP + diphosphate | the enzyme alternates between two conformations during catalysis of the two half-reactions, structure-function analysis, domain alternation strategy, overview | Alcaligenes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Chlorobenzoate + CoA + ATP | - |
Alcaligenes sp. | 4-Chlorobenzoyl-CoA + AMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | determination and analysis of the structure of CBL in the adenylate forming conformation bound to 4-chlorobenzoate and the adenylate-forming conformation bound to the adenylate intermediate, overview | Alcaligenes sp. |
Synonyms | Comment | Organism |
---|---|---|
4-chlorobenzoate:CoA ligase | - |
Alcaligenes sp. |
CBL | - |
Alcaligenes sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Alcaligenes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Alcaligenes sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Alcaligenes sp. |