Cloned (Comment) | Organism |
---|---|
gene phlC, cloning from DNA library in lambda ZAP II, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His8-tagged enzyme in Escherichia coli strain BL21 (DE3)-RP, subcloning in Escherichia coli strain DH5alpha, real-time quantitative RT-PCR expression analysis | Penicillium chrysogenum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Penicillium chrysogenum | |
0.026 | - |
caproate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.046 | - |
caprylate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.07 | - |
caprate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.092 | - |
palmitate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.257 | - |
Phenoxyacetate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.809 | - |
phenylacetate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.841 | - |
stearate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | the enzyme contains a peroxisomal targeting signal 1, A-K-L, on its C terminus, indicating its peroxisomal location | Penicillium chrysogenum | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Penicillium chrysogenum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61635 | - |
x * 61635, sequence calculation | Penicillium chrysogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phenylacetate + CoA | Penicillium chrysogenum | - |
AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | Penicillium chrysogenum WIS 54-1255 | - |
AMP + diphosphate + phenylacetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | D3GE78 | gene phlC | - |
Penicillium chrysogenum WIS 54-1255 | D3GE78 | gene phlC | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 (DE3)-RP by nickel affinity chromatography | Penicillium chrysogenum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00013 | - |
purified recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | reaction of EC 6.2.1.12 | Penicillium chrysogenum | AMP + diphosphate + 4-coumaryl-CoA | - |
? | |
ATP + caprate + CoA | - |
Penicillium chrysogenum | AMP + diphosphate + capryl-CoA | - |
? | |
ATP + caproate + CoA | best substrate | Penicillium chrysogenum | AMP + diphosphate + caproyl-CoA | - |
? | |
ATP + caprylate + CoA | - |
Penicillium chrysogenum | AMP + diphosphate + caprylyl-CoA | - |
? | |
ATP + cinnamate + CoA | reaction of EC 6.2.1.12 | Penicillium chrysogenum | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
ATP + cinnamate + CoA | reaction of EC 6.2.1.12 | Penicillium chrysogenum WIS 54-1255 | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
ATP + palmitate + CoA | low activity, reaction of EC 6.2.1.3 | Penicillium chrysogenum | AMP + diphosphate + palmityl-CoA | - |
? | |
ATP + phenoxyacetate + CoA | low activity | Penicillium chrysogenum | AMP + diphosphate + phenoxyacetyl-CoA | - |
? | |
ATP + phenoxyacetate + CoA | low activity | Penicillium chrysogenum WIS 54-1255 | AMP + diphosphate + phenoxyacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | - |
Penicillium chrysogenum | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | low activity | Penicillium chrysogenum | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | - |
Penicillium chrysogenum WIS 54-1255 | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | low activity | Penicillium chrysogenum WIS 54-1255 | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + stearate + CoA | very low activity, reaction of EC 6.2.1.3 | Penicillium chrysogenum | AMP + diphosphate + stearyl-CoA | - |
? | |
additional information | the enzyme displays broad substrate spectrum and shows higher activities to medium and long-chain fatty acids. The enzyme is also active with cinnamic acid and coumaric acid, see for EC 6.2.1.12 | Penicillium chrysogenum | ? | - |
? | |
additional information | the enzyme displays broad substrate spectrum and shows higher activities to medium and long-chain fatty acids. The enzyme is also active with cinnamic acid and coumaric acid, see for EC 6.2.1.12 | Penicillium chrysogenum WIS 54-1255 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61635, sequence calculation | Penicillium chrysogenum |
Synonyms | Comment | Organism |
---|---|---|
PAA-CoA ligase | - |
Penicillium chrysogenum |
PhlC | - |
Penicillium chrysogenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Penicillium chrysogenum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
palmitate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.062 | - |
stearate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.063 | - |
Phenoxyacetate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.073 | - |
caprylate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.076 | - |
caproate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.099 | - |
phenylacetate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.102 | - |
caprate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Penicillium chrysogenum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Penicillium chrysogenum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Penicillium chrysogenum | sequence calculation | - |
7.66 |
Organism | Comment | Expression |
---|---|---|
Penicillium chrysogenum | the enzyme is induced by phenylacetate | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
stearate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.124 | - |
phenylacetate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.247 | - |
Phenoxyacetate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
0.283 | - |
palmitate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
1.455 | - |
caprate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
1.575 | - |
caprylate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum | |
2.917 | - |
caproate | recombinant enzyme, pH 7.5, 30°C | Penicillium chrysogenum |