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Literature summary for 6.2.1.30 extracted from

  • Yu, Z.L.; Liu, J.; Wang, F.Q.; Dai, M.; Zhao, B.H.; He, J.G.; Zhang, H.
    Cloning and characterization of a novel CoA-ligase gene from Penicillium chrysogenum (2011), Folia Microbiol. (Praha), 56, 246-252.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene phlC, cloning from DNA library in lambda ZAP II, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His8-tagged enzyme in Escherichia coli strain BL21 (DE3)-RP, subcloning in Escherichia coli strain DH5alpha, real-time quantitative RT-PCR expression analysis Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Penicillium chrysogenum
0.026
-
caproate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.046
-
caprylate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.07
-
caprate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.092
-
palmitate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.257
-
Phenoxyacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.809
-
phenylacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.841
-
stearate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome the enzyme contains a peroxisomal targeting signal 1, A-K-L, on its C terminus, indicating its peroxisomal location Penicillium chrysogenum 5777
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Penicillium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61635
-
x * 61635, sequence calculation Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + phenylacetate + CoA Penicillium chrysogenum
-
AMP + diphosphate + phenylacetyl-CoA
-
?
ATP + phenylacetate + CoA Penicillium chrysogenum WIS 54-1255
-
AMP + diphosphate + phenylacetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum D3GE78 gene phlC
-
Penicillium chrysogenum WIS 54-1255 D3GE78 gene phlC
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 (DE3)-RP by nickel affinity chromatography Penicillium chrysogenum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00013
-
purified recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA reaction of EC 6.2.1.12 Penicillium chrysogenum AMP + diphosphate + 4-coumaryl-CoA
-
?
ATP + caprate + CoA
-
Penicillium chrysogenum AMP + diphosphate + capryl-CoA
-
?
ATP + caproate + CoA best substrate Penicillium chrysogenum AMP + diphosphate + caproyl-CoA
-
?
ATP + caprylate + CoA
-
Penicillium chrysogenum AMP + diphosphate + caprylyl-CoA
-
?
ATP + cinnamate + CoA reaction of EC 6.2.1.12 Penicillium chrysogenum AMP + diphosphate + cinnamoyl-CoA
-
?
ATP + cinnamate + CoA reaction of EC 6.2.1.12 Penicillium chrysogenum WIS 54-1255 AMP + diphosphate + cinnamoyl-CoA
-
?
ATP + palmitate + CoA low activity, reaction of EC 6.2.1.3 Penicillium chrysogenum AMP + diphosphate + palmityl-CoA
-
?
ATP + phenoxyacetate + CoA low activity Penicillium chrysogenum AMP + diphosphate + phenoxyacetyl-CoA
-
?
ATP + phenoxyacetate + CoA low activity Penicillium chrysogenum WIS 54-1255 AMP + diphosphate + phenoxyacetyl-CoA
-
?
ATP + phenylacetate + CoA
-
Penicillium chrysogenum AMP + diphosphate + phenylacetyl-CoA
-
?
ATP + phenylacetate + CoA low activity Penicillium chrysogenum AMP + diphosphate + phenylacetyl-CoA
-
?
ATP + phenylacetate + CoA
-
Penicillium chrysogenum WIS 54-1255 AMP + diphosphate + phenylacetyl-CoA
-
?
ATP + phenylacetate + CoA low activity Penicillium chrysogenum WIS 54-1255 AMP + diphosphate + phenylacetyl-CoA
-
?
ATP + stearate + CoA very low activity, reaction of EC 6.2.1.3 Penicillium chrysogenum AMP + diphosphate + stearyl-CoA
-
?
additional information the enzyme displays broad substrate spectrum and shows higher activities to medium and long-chain fatty acids. The enzyme is also active with cinnamic acid and coumaric acid, see for EC 6.2.1.12 Penicillium chrysogenum ?
-
?
additional information the enzyme displays broad substrate spectrum and shows higher activities to medium and long-chain fatty acids. The enzyme is also active with cinnamic acid and coumaric acid, see for EC 6.2.1.12 Penicillium chrysogenum WIS 54-1255 ?
-
?

Subunits

Subunits Comment Organism
? x * 61635, sequence calculation Penicillium chrysogenum

Synonyms

Synonyms Comment Organism
PAA-CoA ligase
-
Penicillium chrysogenum
PhlC
-
Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Penicillium chrysogenum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.026
-
palmitate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.062
-
stearate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.063
-
Phenoxyacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.073
-
caprylate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.076
-
caproate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.099
-
phenylacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.102
-
caprate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Penicillium chrysogenum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Penicillium chrysogenum

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium chrysogenum sequence calculation
-
7.66

Expression

Organism Comment Expression
Penicillium chrysogenum the enzyme is induced by phenylacetate up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.074
-
stearate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.124
-
phenylacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.247
-
Phenoxyacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
0.283
-
palmitate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
1.455
-
caprate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
1.575
-
caprylate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
2.917
-
caproate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum