Cloned (Comment) | Organism |
---|---|
- |
Aromatoleum evansii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, more than 80% inhibition | Aromatoleum evansii | |
iodoacetamide | - |
Aromatoleum evansii | |
NaF | 2 mM; 2 mM, 84% loss of activity | Aromatoleum evansii | |
NEM | - |
Aromatoleum evansii | |
Ni2+ | 1 mM, more than 80% inhibition | Aromatoleum evansii | |
Zn2+ | 1 mM, more than 80% inhibition | Aromatoleum evansii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
phenylacetate | 37°C | Aromatoleum evansii | |
0.045 | - |
CoA | 37°C | Aromatoleum evansii | |
0.06 | - |
ATP | 37°C | Aromatoleum evansii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Aromatoleum evansii | |
Mn2+ | 36% of the activation with Mg2+ | Aromatoleum evansii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48750 | - |
x * 48750, calculation from nucleotide sequence | Aromatoleum evansii |
50000 | - |
1 * 50000, SDS-PAGE | Aromatoleum evansii |
52000 | - |
gel filtration | Aromatoleum evansii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phenylacetate + CoA | Aromatoleum evansii | first enzyme involved in the aerobic catabolism of phenylacetic acid | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aromatoleum evansii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aromatoleum evansii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000076 | - |
maximal specific activity in growing cultures | Aromatoleum evansii |
0.48 | - |
- |
Aromatoleum evansii |
Storage Stability | Organism |
---|---|
-20°C, 10% glycerol, activity is retained for 2 months without remarkable loss of activity | Aromatoleum evansii |
4°C, 10% glycerol, 65% of the activity is recovered after 72 h | Aromatoleum evansii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phenylacetate + CoA | - |
Aromatoleum evansii | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | first enzyme involved in the aerobic catabolism of phenylacetic acid | Aromatoleum evansii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Aromatoleum evansii |
monomer | x * 48750, calculation from nucleotide sequence | Aromatoleum evansii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
ATP | 37°C | Aromatoleum evansii | |
40 | - |
CoA | 37°C | Aromatoleum evansii | |
40 | - |
phenylacetate | 37°C | Aromatoleum evansii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Aromatoleum evansii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 9: 55% of maximal activity, pH 9.0: 50% of maximal activity | Aromatoleum evansii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aromatoleum evansii | isoelectric focusing | - |
6.1 |