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Literature summary for 6.2.1.25 extracted from

  • Altenschmidt, U.; Oswald, B.; Fuchs, G.
    Purification and characterization of benzoate-coenzyme A ligase and 2-aminobenzoate-coenzyme A ligase from a denitrifying Pseudomonas sp. (1991), J. Bacteriol., 173, 5494-5501.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.25

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Pseudomonas sp.
CuCl2
-
 Pseudomonas sp.
n-octylglucoside weak Pseudomonas sp.
p-chloromercuribenzoate
-
 Pseudomonas sp.
SDS
-
 Pseudomonas sp.
Tween 100
-
 Pseudomonas sp.
Zn2+
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 benzoate
-
 Pseudomonas sp.
0.1
-
 CoASH CoA Pseudomonas sp.
0.1
-
 CoASH 2-fluorobenzoate Pseudomonas sp.
0.13
-
 ATP
-
 Pseudomonas sp.
0.25
-
 2-Aminobenzoate
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas sp.
Mn2+ can replace Mg2+ Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. KB740
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-aminobenzoate + CoA 28% of the activity relative to benzoate Pseudomonas sp. AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
ATP + 2-aminobenzoate + CoA 28% of the activity relative to benzoate Pseudomonas sp. KB740 AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
ATP + 2-fluorobenzoate + CoA 120% of the activity relative to benzoate Pseudomonas sp. AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
ATP + 2-fluorobenzoate + CoA 120% of the activity relative to benzoate Pseudomonas sp. KB740 AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
ATP + 3-fluorobenzoate + CoA 76% of the activity relative to benzoate Pseudomonas sp. AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
ATP + 3-fluorobenzoate + CoA 76% of the activity relative to benzoate Pseudomonas sp. KB740 AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
ATP + 4-fluorobenzoate + CoA as effective as benzoate Pseudomonas sp. AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
ATP + 4-fluorobenzoate + CoA as effective as benzoate Pseudomonas sp. KB740 AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
ATP + benzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + benzoyl-CoA
-
 ?
ATP + benzoate + CoA
-
 Pseudomonas sp. KB740 AMP + diphosphate + benzoyl-CoA
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
433
-
 benzoate
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
 Pseudomonas sp.