General Stability | Organism |
---|---|
stabilized by nucleotides, most potent stabilizer is ADP | Rubrivivax gelatinosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000037 | - |
citrate lyase | sulfhydryl form | Rubrivivax gelatinosus | |
0.14 | - |
ATP | - |
Rubrivivax gelatinosus | |
5 | - |
acetate | - |
Rubrivivax gelatinosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal concentration: 3 mM, inhibitory above 5 mM | Rubrivivax gelatinosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37500 | - |
gel filtration, density gradient centrifugation | Rubrivivax gelatinosus |
41690 | - |
1 * 41690, SDS-PAGE | Rubrivivax gelatinosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rubrivivax gelatinosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
copurification of citrate lyase and citrate lyase ligase | Rubrivivax gelatinosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rubrivivax gelatinosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase | Rubrivivax gelatinosus | AMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
ATP + acetate + citrate (pro-3S)-lyase | highly specific, cannot react with citrate lyases from nonphototrophic microorganisms, e.g. Enterobacter aerogenes, Clostridium sphenoides or Streptococcus diacetilactis | Rubrivivax gelatinosus | AMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
ATP + acetate + citrate lyase | - |
Rubrivivax gelatinosus | AMP + diphosphate + acetyl-[citrate lyase] | - |
? | |
ATP + propionate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, propionate at 47% of the activity relative to acetate | Rubrivivax gelatinosus | AMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
CTP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, CTP at 30% of the relative to ATP | Rubrivivax gelatinosus | CMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
dTTP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, dTTP at 5% of the activity relative to ATP | Rubrivivax gelatinosus | dTMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
GTP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, GTP at 43% of the activity of ATP | Rubrivivax gelatinosus | GMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
ITP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, ITP at 46% of the activity relative to ATP | Rubrivivax gelatinosus | IMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
UTP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, UTP at 18% of the activity relative to ATP | Rubrivivax gelatinosus | UMP + diphosphate + citrate(pro-3S)-lyase | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41690, SDS-PAGE | Rubrivivax gelatinosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Rubrivivax gelatinosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 6: 50% of maximal activity, 8: 55% of maximal activity | Rubrivivax gelatinosus |