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Literature summary for 6.2.1.20 extracted from
- Purification and characterization of acyl-acyl carrier protein synthetase from oleaginous yeast and its role in triacylglycerol biosynthesis (2001), Biochem. J., 360, 471-479.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
linoleic acid | pH 8.0, 30°C | Rhodotorula glutinis |  |
| 0.025 | - |
oleic acid | pH 8.0, 30°C | Rhodotorula glutinis | |
| 0.03 | - |
stearic acid | pH 8.0, 30°C | Rhodotorula glutinis | |
| 0.043 | - |
palmitic acid | pH 8.0, 30°C | Rhodotorula glutinis | |
| 2.5 | - |
ATP | pH 8.0, 30°C | Rhodotorula glutinis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rhodotorula glutinis | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE and native PAGE | Rhodotorula glutinis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + an acid + [acyl-carrier protein] | Rhodotorula glutinis | synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates and donor for activated acyl groups for the synthesis of lipids and lipid derivatives | AMP + diphosphate + acyl-[acyl-carrier protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula glutinis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodotorula glutinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + an acid + [acyl-carrier protein] | - |
Rhodotorula glutinis | AMP + diphosphate + acyl-[acyl-carrier protein] | - |
? | |
| ATP + an acid + [acyl-carrier protein] | synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates and donor for activated acyl groups for the synthesis of lipids and lipid derivatives | Rhodotorula glutinis | AMP + diphosphate + acyl-[acyl-carrier protein] | - |
? | |
| ATP + linoleic acid + [acyl-carrier protein] | most effective subtrate | Rhodotorula glutinis | AMP + diphosphate + linoleoyl-[acyl-carrier protein] | - |
? | |
| ATP + oleic acid + [acyl-carrier protein] | - |
Rhodotorula glutinis | AMP + diphosphate + oleoyl-[acyl-carrier protein] | - |
? | |
| ATP + palmitic acid + [acyl-carrier protein] | - |
Rhodotorula glutinis | AMP + diphosphate + palmitoyl-[acyl-carrier protein] | - |
? | |
| ATP + stearic acid + [acyl-carrier protein] | - |
Rhodotorula glutinis | AMP + diphosphate + stearoyl-[acyl-carrier protein] | - |
? | |
| additional information | no activity with GTP, CTP or UTP | Rhodotorula glutinis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, molecular weight of native protein and subunits by native and SDS-PAGE | Rhodotorula glutinis |
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