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Literature summary for 6.2.1.2 extracted from

  • Webster, L.T.; Gerowin, L.D.; Rakita, L.
    Purification and characteristics of a butyryl coenzyme A synthetase from bovine heart mitochondria (1965), J. Biol. Chem., 240, 29-33.
    View publication on PubMed
Search for more literature for 6.2.1.2

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4
-
 Bos taurus
p-chloromercuribenzoate butyrate or ATP partially protects Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.85
-
 CoA
-
 Bos taurus
1.5
-
 Butyrate
-
 Bos taurus
3
-
 ATP
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bos taurus
Mg2+ Km: 1.8 mM Bos taurus
Mn2+ can replace Mg2+ in activation Bos taurus
additional information no differential response to monovalent cations Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + butyrate + CoA no activity with GTP Bos taurus AMP + diphosphate + butyryl-CoA
-
 ?
ATP + crotonate + CoA i.e. E-2-butenoate, 20% of the activity relative to butyrate Bos taurus AMP + diphosphate + crotonyl-CoA
-
 ?
ATP + isobutyrate + CoA 20% of the activity relative to butyrate Bos taurus AMP + diphosphate + isobutyryl-CoA
-
 ?
CTP + butyrate + CoA slight activity Bos taurus CMP + diphosphate + butyryl-CoA
-
 ?
ITP + butyrate + CoA slight activity Bos taurus IMP + diphosphate + butyryl-CoA
-
 ?
additional information no activity with formate and heptanoate Bos taurus ?
-
 ?
additional information straight chain acids having 3, 5 or 6 carbons are less active as substrates than butyrate Bos taurus ?
-
 ?
additional information no activity with acetate and octanoate Bos taurus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 broad pH-optimum around Bos taurus