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Literature summary for 6.2.1.2 extracted from

  • Takao, S.; Ito, T.; Tanida, M.
    Purification and kinetic properties of butyryl-CoA synthetase from Paecilomyces varioti (1987), Agric. Biol. Chem., 51, 145-152.
No PubMed abstract available
Search for more literature for 6.2.1.2

Inhibitors

Inhibitors Comment Organism Structure
AMP competitive for ATP Paecilomyces variotii
butyryl-CoA competitive for ATP Paecilomyces variotii
diphosphate competitive for CoA Paecilomyces variotii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 CoA
-
 Paecilomyces variotii
0.14
-
 Butyrate ATP Paecilomyces variotii
0.38
-
 pentanoate
-
 Paecilomyces variotii
0.47
-
 Isopentanoate
-
 Paecilomyces variotii
1.6
-
 Isobutyrate
-
 Paecilomyces variotii
3.3
-
 propionate
-
 Paecilomyces variotii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can replace Mg2+ in activation with 47% of the efficiency, Km: 0.14 mM Paecilomyces variotii
K+ requires both Mg2+ and K+, Km: 2.4 mM Paecilomyces variotii
Mg2+ requires both Mg2+ and K+ Paecilomyces variotii
Mn2+ can replace Mg2+ in activation Paecilomyces variotii
Mn2+ with 128% of the activation compared to Mg2+, Km: 0.14 mM Paecilomyces variotii
Rb+ can replace K+ in activation with 81% efficiency, Km: 2.4 mM Paecilomyces variotii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 2 * 74000, SDS-PAGE Paecilomyces variotii
145000
-
 gel filtration Paecilomyces variotii

Organism

Organism UniProt Comment Textmining
Paecilomyces variotii
-
-
-
Paecilomyces variotii AHU 9417
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paecilomyces variotii

Reaction

Reaction Comment Organism Reaction ID
ATP + a medium-chain fatty acid + CoA = AMP + diphosphate + a medium-chain acyl-CoA bi uni uni bi ping-pong mechanism, in which the order of addition and evolution of substrates and products is ATP, butyrate, diphosphate, CoA, butyryl-CoA or AMP, and AMP or butyryl-CoA. Butyryl-CoA and AMP are evolved at random or at the same time Paecilomyces variotii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Paecilomyces variotii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + butyrate + CoA
-
 Paecilomyces variotii AMP + diphosphate + butyryl-CoA
-
 ?
ATP + butyrate + CoA
-
 Paecilomyces variotii AHU 9417 AMP + diphosphate + butyryl-CoA
-
 ?
ATP + isobutyrate + CoA
-
 Paecilomyces variotii AMP + diphosphate + isobutyryl-CoA
-
 ?
ATP + isopentanoate
-
 Paecilomyces variotii AMP + diphosphate + isopentanoyl-CoA
-
 ?
ATP + pentanoate + CoA
-
 Paecilomyces variotii AMP + diphosphate + pentanoyl-CoA
-
 ?
ATP + pentanoate + CoA
-
 Paecilomyces variotii AHU 9417 AMP + diphosphate + pentanoyl-CoA
-
 ?
ATP + propionate + CoA
-
 Paecilomyces variotii AMP + diphosphate + propionyl-CoA
-
 ?
ATP + propionate + CoA
-
 Paecilomyces variotii AHU 9417 AMP + diphosphate + propionyl-CoA
-
 ?
additional information no activity with hexanoate Paecilomyces variotii ?
-
 ?
additional information no activity with acetate and octanoate Paecilomyces variotii ?
-
 ?
additional information no activity with hexanoate Paecilomyces variotii AHU 9417 ?
-
 ?
additional information no activity with acetate and octanoate Paecilomyces variotii AHU 9417 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 74000, SDS-PAGE Paecilomyces variotii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Paecilomyces variotii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Paecilomyces variotii