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Literature summary for 6.2.1.14 extracted from

  • Binieda, A.; Fuhrmann, M.; Lehner, B.; Rey-Berthod, C.; Frutiger-Hughes, S.; Hughes, G.; Shaw, N.M.
    Purification, characterization, DNA sequence and cloning of a pimeloyl-CoA synthetase from Pseudomonas mendocina 35 (1999), Biochem. J., 340, 793-801.
No PubMed abstract available
Search for more literature for 6.2.1.14

Application

Application Comment Organism
synthesis production of biotin Pseudomonas mendocina

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Pseudomonas mendocina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetic Pseudomonas mendocina
0.18
-
 CoA pH 8.0, 30ºC Pseudomonas mendocina
0.49
-
 heptanedioate pH 8.0, 30ºC Pseudomonas mendocina
0.72
-
 ATP pH 8.0, 30ºC Pseudomonas mendocina

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Pseudomonas mendocina
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74643
-
 4 * 74643, calculation from sequence of cDNA Pseudomonas mendocina
298600
-
 calculated from sequence of cDNA Pseudomonas mendocina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + heptanedioate + CoA Pseudomonas mendocina first enzyme of biotin biosynthesis pathway AMP + diphosphate + heptanedioyl-CoA
-
 ?
ATP + heptanedioate + CoA Pseudomonas mendocina 35 first enzyme of biotin biosynthesis pathway AMP + diphosphate + heptanedioyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina Q9ZER2
-
-
Pseudomonas mendocina 35 Q9ZER2
-
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange, gel filtration, chromatofocusing Pseudomonas mendocina

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Pseudomonas mendocina
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
77.3
-
 freshly prepared enzyme, pH 8.0, at 30ºC Pseudomonas mendocina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + heptanedioate + CoA no activity with ethanedioate and pentanedioate Pseudomonas mendocina AMP + diphosphate + heptanedioyl-CoA
-
 ?
ATP + heptanedioate + CoA first enzyme of biotin biosynthesis pathway Pseudomonas mendocina AMP + diphosphate + heptanedioyl-CoA
-
 ?
ATP + heptanedioate + CoA no activity with ethanedioate and pentanedioate Pseudomonas mendocina 35 AMP + diphosphate + heptanedioyl-CoA
-
 ?
ATP + heptanedioate + CoA first enzyme of biotin biosynthesis pathway Pseudomonas mendocina 35 AMP + diphosphate + heptanedioyl-CoA
-
 ?
ATP + hexanedioate + CoA 72% activity of that for heptanedioate Pseudomonas mendocina AMP + diphosphate + hexanedioyl-CoA
-
 ?
ATP + hexanedioate + CoA 72% activity of that for heptanedioate Pseudomonas mendocina 35 AMP + diphosphate + hexanedioyl-CoA
-
 ?
ATP + nonanedioate + CoA 18% activity of that for heptanedioate Pseudomonas mendocina AMP + diphosphate + nonanedioyl-CoA
-
 ?
ATP + nonanedioate + CoA 18% activity of that for heptanedioate Pseudomonas mendocina 35 AMP + diphosphate + nonanedioyl-CoA
-
 ?

Subunits

Subunits Comment Organism
More subunit: estimated to be between 74000 and 80000, SDS-PAGE Pseudomonas mendocina
tetramer 4 * 74643, calculation from sequence of cDNA Pseudomonas mendocina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 approximately, this value should be considered as a functional pH optimum because of the complex assay system 30ºC Pseudomonas mendocina

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas mendocina pH-gradient 6.0-4.0, chromatofocusing
-
 5.3