Application | Comment | Organism |
---|---|---|
synthesis | production of biotin | Pseudomonas mendocina |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Pseudomonas mendocina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetic | Pseudomonas mendocina | |
0.18 | - |
CoA | pH 8.0, 30ºC | Pseudomonas mendocina | |
0.49 | - |
heptanedioate | pH 8.0, 30ºC | Pseudomonas mendocina | |
0.72 | - |
ATP | pH 8.0, 30ºC | Pseudomonas mendocina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Pseudomonas mendocina | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74643 | - |
4 * 74643, calculation from sequence of cDNA | Pseudomonas mendocina |
298600 | - |
calculated from sequence of cDNA | Pseudomonas mendocina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + heptanedioate + CoA | Pseudomonas mendocina | first enzyme of biotin biosynthesis pathway | AMP + diphosphate + heptanedioyl-CoA | - |
? | |
ATP + heptanedioate + CoA | Pseudomonas mendocina 35 | first enzyme of biotin biosynthesis pathway | AMP + diphosphate + heptanedioyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mendocina | Q9ZER2 | - |
- |
Pseudomonas mendocina 35 | Q9ZER2 | - |
- |
Purification (Comment) | Organism |
---|---|
anion exchange, gel filtration, chromatofocusing | Pseudomonas mendocina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pseudomonas mendocina | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
77.3 | - |
freshly prepared enzyme, pH 8.0, at 30ºC | Pseudomonas mendocina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + heptanedioate + CoA | no activity with ethanedioate and pentanedioate | Pseudomonas mendocina | AMP + diphosphate + heptanedioyl-CoA | - |
? | |
ATP + heptanedioate + CoA | first enzyme of biotin biosynthesis pathway | Pseudomonas mendocina | AMP + diphosphate + heptanedioyl-CoA | - |
? | |
ATP + heptanedioate + CoA | no activity with ethanedioate and pentanedioate | Pseudomonas mendocina 35 | AMP + diphosphate + heptanedioyl-CoA | - |
? | |
ATP + heptanedioate + CoA | first enzyme of biotin biosynthesis pathway | Pseudomonas mendocina 35 | AMP + diphosphate + heptanedioyl-CoA | - |
? | |
ATP + hexanedioate + CoA | 72% activity of that for heptanedioate | Pseudomonas mendocina | AMP + diphosphate + hexanedioyl-CoA | - |
? | |
ATP + hexanedioate + CoA | 72% activity of that for heptanedioate | Pseudomonas mendocina 35 | AMP + diphosphate + hexanedioyl-CoA | - |
? | |
ATP + nonanedioate + CoA | 18% activity of that for heptanedioate | Pseudomonas mendocina | AMP + diphosphate + nonanedioyl-CoA | - |
? | |
ATP + nonanedioate + CoA | 18% activity of that for heptanedioate | Pseudomonas mendocina 35 | AMP + diphosphate + nonanedioyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | subunit: estimated to be between 74000 and 80000, SDS-PAGE | Pseudomonas mendocina |
tetramer | 4 * 74643, calculation from sequence of cDNA | Pseudomonas mendocina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
approximately, this value should be considered as a functional pH optimum because of the complex assay system 30ºC | Pseudomonas mendocina |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas mendocina | pH-gradient 6.0-4.0, chromatofocusing | - |
5.3 |