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Literature summary for 6.2.1.14 extracted from

  • Ploux, O.; Soularue, P.; Marquet, A.; Gloeckler, R.; Lemoine, Y.
    Investigation of the first step of biotin biosynthesis in Bacillus sphaericus. Purification and characterization of the pimeloyl-CoA synthetase, and uptake of pimelate (1992), Biochem. J., 287, 685-690.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Lysinibacillus sphaericus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Lysinibacillus sphaericus
EDTA
-
Lysinibacillus sphaericus
Mg2+ inhibition above 10 mM, requirement at lower concentrations Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
CoA
-
Lysinibacillus sphaericus
0.145
-
heptanedioate
-
Lysinibacillus sphaericus
0.17
-
MgATP2-
-
Lysinibacillus sphaericus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 32% of the activation relative to Mg2+ Lysinibacillus sphaericus
Mg2+ required Lysinibacillus sphaericus
Mg2+ Km: 2.3 mM Lysinibacillus sphaericus
Mg2+ inhibition above 10 mM Lysinibacillus sphaericus
Mn2+ 73% of the activation relative to Mg2+ Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
2 * 28000, SDS-PAGE Lysinibacillus sphaericus
60000
-
gel filtration Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-
Lysinibacillus sphaericus
-
expressed in an overproducing Escherichia coli strain C268
-
Lysinibacillus sphaericus IFO 3525
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
-
Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 6-carboxyhexanoate + CoA
-
Lysinibacillus sphaericus AMP + diphosphate + 6-carboxyhexanoyl-CoA
-
?
ATP + 6-carboxyhexanoate + CoA
-
Lysinibacillus sphaericus IFO 3525 AMP + diphosphate + 6-carboxyhexanoyl-CoA
-
?
ATP + heptanedioate + CoA
-
Lysinibacillus sphaericus AMP + diphosphate + heptanedioyl-CoA heptanedioyl-CoA i.e. pimelyl-CoA ?
ATP + heptanedioate + CoA
-
Lysinibacillus sphaericus IFO 3525 AMP + diphosphate + heptanedioyl-CoA heptanedioyl-CoA i.e. pimelyl-CoA ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Lysinibacillus sphaericus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
Lysinibacillus sphaericus

pH Range

pH Minimum pH Maximum Comment Organism
8 9.5 8: about 55% of maximal activity, 9.5: about 65% of maximal activity Lysinibacillus sphaericus