Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Lysinibacillus sphaericus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Lysinibacillus sphaericus | |
EDTA | - |
Lysinibacillus sphaericus | |
Mg2+ | inhibition above 10 mM, requirement at lower concentrations | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
CoA | - |
Lysinibacillus sphaericus | |
0.145 | - |
heptanedioate | - |
Lysinibacillus sphaericus | |
0.17 | - |
MgATP2- | - |
Lysinibacillus sphaericus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 32% of the activation relative to Mg2+ | Lysinibacillus sphaericus | |
Mg2+ | required | Lysinibacillus sphaericus | |
Mg2+ | Km: 2.3 mM | Lysinibacillus sphaericus | |
Mg2+ | inhibition above 10 mM | Lysinibacillus sphaericus | |
Mn2+ | 73% of the activation relative to Mg2+ | Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Lysinibacillus sphaericus |
60000 | - |
gel filtration | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Lysinibacillus sphaericus | - |
expressed in an overproducing Escherichia coli strain C268 | - |
Lysinibacillus sphaericus IFO 3525 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
- |
Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 6-carboxyhexanoate + CoA | - |
Lysinibacillus sphaericus | AMP + diphosphate + 6-carboxyhexanoyl-CoA | - |
? | |
ATP + 6-carboxyhexanoate + CoA | - |
Lysinibacillus sphaericus IFO 3525 | AMP + diphosphate + 6-carboxyhexanoyl-CoA | - |
? | |
ATP + heptanedioate + CoA | - |
Lysinibacillus sphaericus | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Lysinibacillus sphaericus IFO 3525 | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE | Lysinibacillus sphaericus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Lysinibacillus sphaericus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | 8: about 55% of maximal activity, 9.5: about 65% of maximal activity | Lysinibacillus sphaericus |