Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Chloroflexus aurantiacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
CoA | in 100 mM HEPES-KOH pH 7.5, 0.1 mM 5,5'-dithiobis(2-nitrobenzoic acid), 5 mM MgCl2, at 55°C | Chloroflexus aurantiacus | |
0.037 | - |
acetyl-CoA | in 100 mM HEPES-KOH pH 7.5, 0.1 mM 5,5'-dithiobis(2-nitrobenzoic acid), 5 mM MgCl2, at 55°C | Chloroflexus aurantiacus | |
0.091 | - |
ADP | in 100 mM HEPES-KOH pH 7.5, 0.1 mM 5,5'-dithiobis(2-nitrobenzoic acid), 5 mM MgCl2, at 55°C | Chloroflexus aurantiacus | |
0.57 | - |
ATP | in 100 mM HEPES-KOH pH 7.5, 0.1 mM 5,5'-dithiobis(2-nitrobenzoic acid), 5 mM MgCl2, at 55°C | Chloroflexus aurantiacus | |
0.9 | - |
acetate | in 100 mM HEPES-KOH pH 7.5, 0.1 mM 5,5'-dithiobis(2-nitrobenzoic acid), 5 mM MgCl2, at 55°C | Chloroflexus aurantiacus | |
1 | - |
phosphate | in 100 mM HEPES-KOH pH 7.5, 0.1 mM 5,5'-dithiobis(2-nitrobenzoic acid), 5 mM MgCl2, at 55°C | Chloroflexus aurantiacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on Mg2+, showing the highest activity at about 5 mM | Chloroflexus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
4 * 75000, native enzyme, SDS-PAGE | Chloroflexus aurantiacus |
77000 | - |
4 * 77000, recombinant enzyme, SDS-PAGE | Chloroflexus aurantiacus |
300000 | - |
native enzyme, gel filtration | Chloroflexus aurantiacus |
317000 | - |
recombinant enzyme, gel filtration | Chloroflexus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphate + acetyl-CoA | Chloroflexus aurantiacus | - |
ATP + acetate + CoA | - |
r | |
ADP + phosphate + acetyl-CoA | Chloroflexus aurantiacus J-10-fl | - |
ATP + acetate + CoA | - |
r | |
ATP + acetate + CoA | Chloroflexus aurantiacus | - |
ADP + phosphate + acetyl-CoA | - |
r | |
ATP + acetate + CoA | Chloroflexus aurantiacus J-10-fl | - |
ADP + phosphate + acetyl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | - |
- |
- |
Chloroflexus aurantiacus J-10-fl | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity chromatography, phenyl Sepharose column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration | Chloroflexus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphate + acetyl-CoA | - |
Chloroflexus aurantiacus | ATP + acetate + CoA | - |
r | |
ADP + phosphate + acetyl-CoA | - |
Chloroflexus aurantiacus J-10-fl | ATP + acetate + CoA | - |
r | |
ATP + 2-methylbutyrate + CoA | 75% activity compared to acetate | Chloroflexus aurantiacus | ADP + phosphate + 2-methylbutyryl-CoA | - |
? | |
ATP + acetate + CoA | - |
Chloroflexus aurantiacus | ADP + phosphate + acetyl-CoA | - |
r | |
ATP + acetate + CoA | 100% activity | Chloroflexus aurantiacus | ADP + phosphate + acetyl-CoA | - |
r | |
ATP + acetate + CoA | - |
Chloroflexus aurantiacus J-10-fl | ADP + phosphate + acetyl-CoA | - |
r | |
ATP + butyrate + CoA | 98% activity compared to acetate | Chloroflexus aurantiacus | ADP + phosphate + butyryl-CoA | - |
? | |
ATP + imidazole-4-acetate + CoA | 17% activity compared to acetate | Chloroflexus aurantiacus | ADP + phosphate + imidazol-4-ylacetyl-CoA | - |
? | |
ATP + isobutyrate + CoA | 46% activity compared to acetate | Chloroflexus aurantiacus | ADP + phosphate + isobutyryl-CoA | - |
? | |
ATP + isobutyrate + CoA | 46% activity compared to acetate | Chloroflexus aurantiacus J-10-fl | ADP + phosphate + isobutyryl-CoA | - |
? | |
ATP + isovalerate + CoA | 65% activity compared to acetate | Chloroflexus aurantiacus | ADP + phosphate + isovaleryl-CoA | - |
? | |
ATP + phenylacetate + CoA | 10% activity compared to acetate | Chloroflexus aurantiacus | ADP + phosphate + phenylacetyl-CoA | - |
? | |
ATP + propionate + CoA | 100% activity compared to acetate | Chloroflexus aurantiacus | ADP + phosphate + propionyl-CoA | - |
? | |
ATP + propionate + CoA | 100% activity compared to acetate | Chloroflexus aurantiacus J-10-fl | ADP + phosphate + propionyl-CoA | - |
? | |
ATP + thioglycolate + CoA | 110% activity compared to acetate | Chloroflexus aurantiacus | ADP + phosphate + thioglycolyl-CoA | - |
? | |
additional information | 4-hydroxyphenylacetate, indol-3-acetate and succinate are not utilized as substrates | Chloroflexus aurantiacus | ? | - |
? | |
additional information | 4-hydroxyphenylacetate, indol-3-acetate and succinate are not utilized as substrates | Chloroflexus aurantiacus J-10-fl | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 75000, native enzyme, SDS-PAGE | Chloroflexus aurantiacus |
homotetramer | 4 * 77000, recombinant enzyme, SDS-PAGE | Chloroflexus aurantiacus |
Synonyms | Comment | Organism |
---|---|---|
ACD | - |
Chloroflexus aurantiacus |
Acetyl-CoA synthetase (ADP-forming) | - |
Chloroflexus aurantiacus |
ADP-forming acetyl-CoA synthetase isoenzyme I | - |
Chloroflexus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Chloroflexus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Chloroflexus aurantiacus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 7.8 | the pH optimum is at pH 7.5, with remaining activities of about 40% at pH 7.3 and 59% at pH 7.8 | Chloroflexus aurantiacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Chloroflexus aurantiacus |
Organism | Comment | Expression |
---|---|---|
Chloroflexus aurantiacus | the enzyme is induced 10fold during growth on D-glucose | up |