Any feedback?
Literature summary for 6.2.1.13 extracted from
- Unusual ADP-forming acetyl-coenzyme A synthetases from the mesophilic halophilic euryarchaeon Haloarcula marismortui and from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum (2004), Arch. Microbiol., 182, 277-287.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Haloarcula marismortui |
| expression in Escherichia coli | Pyrobaculum aerophilum |
General Stability
| General Stability | Organism |
|---|---|
| requires high salt concentrations for long-term stability | Haloarcula marismortui |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Isobutyrate | 10 mM, complete inhibition | Haloarcula marismortui |  |
| NaCl | 2-3 M, 40% loss of activity | Haloarcula marismortui | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
isobutyryl-CoA | pH 6.5, 85°C | Pyrobaculum aerophilum | |
| 0.046 | - |
ADP | pH 7.5, 37°C | Haloarcula marismortui | |
| 0.08 | - |
CoA | pH 7.5, 37°C | Haloarcula marismortui | |
| 0.11 | - |
phenylacetyl-CoA | pH 6.5, 85°C | Pyrobaculum aerophilum | |
| 0.12 | - |
acetyl-CoA | pH 6.5, 85°C | Pyrobaculum aerophilum | |
| 0.3 | - |
ATP | pH 7.5, 37°C | Haloarcula marismortui | |
| 0.45 | - |
acetyl-CoA | pH 7.5, 37°C | Haloarcula marismortui | |
| 1.1 | - |
acetate | pH 7.5, 37°C | Haloarcula marismortui | |
| 2 | - |
phosphate | pH 7.5, 37°C | Haloarcula marismortui | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal MgCl2 concentration: 30 mM in absence of KCl, 20 mM in presence of 3 M KCl | Haloarcula marismortui | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87000 | - |
2 * 87000, SDS-PAGE | Haloarcula marismortui |
| 166000 | - |
gel filtration | Haloarcula marismortui |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphate + acetyl-CoA | Haloarcula marismortui | - |
ATP + acetate + CoA | - |
r | |
| ADP + phosphate + acetyl-CoA | Pyrobaculum aerophilum | - |
ATP + acetate + CoA | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloarcula marismortui | - |
- |
- |
| Pyrobaculum aerophilum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haloarcula marismortui |
- |
Pyrobaculum aerophilum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Haloarcula marismortui |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphate + acetyl-CoA | - |
Haloarcula marismortui | ATP + acetate + CoA | - |
r | |
| ADP + phosphate + acetyl-CoA | - |
Pyrobaculum aerophilum | ATP + acetate + CoA | - |
r | |
| ADP + phosphate + isobutyryl-CoA | - |
Pyrobaculum aerophilum | ATP + isobutyrate + CoA | - |
r | |
| ADP + phosphate + phenylacetyl-CoA | - |
Pyrobaculum aerophilum | ATP + phenylacetate + CoA | - |
r | |
| ATP + acetate + CoA | - |
Haloarcula marismortui | ADP + phosphate + acetyl-CoA | - |
r | |
| ATP + butyrate + CoA | at 98% of the activity with acetate | Haloarcula marismortui | ADP + phosphate + butyryl-CoA | - |
? | |
| ATP + isobutyrate + CoA | at 38% of the activity with acetate | Haloarcula marismortui | ADP + phosphate + isobutyryl-CoA | - |
? | |
| ATP + isovalerate + CoA | at 25% of the activity with acetate | Haloarcula marismortui | ADP + phosphate + isovaleryl-CoA | - |
? | |
| ATP + propionate + CoA | at 108% of the activity with acetate | Haloarcula marismortui | ADP + phosphate + propionyl-CoA | - |
? | |
| GTP + acetate + CoA | 11% of the activity with ATP | Haloarcula marismortui | GDP + phosphate + acetyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 87000, SDS-PAGE | Haloarcula marismortui |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43 | - |
- |
Haloarcula marismortui |
| 93 | - |
above | Pyrobaculum aerophilum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 30°C: about 50% of maximal activity, 50°C: about 80% of maximal activity | Haloarcula marismortui |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
120 min, no significant loss of activity | Pyrobaculum aerophilum |
| 90 | - |
half-life: 105 min | Pyrobaculum aerophilum |
| 100 | - |
half-life: 13 min. Completely stable for 120 min in presence of 1 M (NH4)2SO4 | Pyrobaculum aerophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Pyrobaculum aerophilum |
| 7.5 | - |
- |
Haloarcula marismortui |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | pH 5.5: about 40% of maximal activity, pH 7.5: about 20% of maximal activity | Pyrobaculum aerophilum |
| 6.5 | 8.5 | pH 6.5: about 60% of maximal activity, pH 8.5: about 60% of maximal activity | Haloarcula marismortui |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
