Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, quantitative real-time PCR expression analysis | Hibiscus cannabinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59560 | - |
x * 59560, sequence calculation | Hibiscus cannabinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hibiscus cannabinus | T1RK64 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | mature, low expression level | Hibiscus cannabinus | - |
leaf | low expression level | Hibiscus cannabinus | - |
additional information | differential expression in all tissues during the developmental stages, transcript expression patterns, quantitative real-time PCR analysis, overview | Hibiscus cannabinus | - |
root | high expression level | Hibiscus cannabinus | - |
stem | high expression level | Hibiscus cannabinus | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 59560, sequence calculation | Hibiscus cannabinus |
Synonyms | Comment | Organism |
---|---|---|
4-coumarate: coenzyme A ligase | - |
Hibiscus cannabinus |
4CL | - |
Hibiscus cannabinus |
Hc4CL | - |
Hibiscus cannabinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Hibiscus cannabinus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hibiscus cannabinus | sequence calculation | - |
6.58 |
Organism | Comment | Expression |
---|---|---|
Hibiscus cannabinus | the enzyme expression is reduced by abscisic acid treatment. The Hc4CL gene is downregulated at early time points and then upregulated 24 h after H2O2 treatment, after which the transcript level returns to a negative level relative to the control. The enzyme also shows biphasic expression in response to wounding. Specifically, the transcript is highly expressed at 6 h and then decreases sharply at 12 h with a second induction at 24 h | down |
Hibiscus cannabinus | Hc4CL is upregulated by NaCl treatment, reaching its maximum level at 24 h after treatment. The enzyme is induced by cold and drought treatment. The Hc4CL gene is downregulated at early time points and then upregulated 24 h after H2O2 treatment, after which the transcript level returns to a negative level relative to the control. The enzyme also shows biphasic expression in response to wounding. Specifically, the transcript is highly expressed at 6 h and then decreases sharply at 12 h with a second induction at 24 h | up |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme has two conserved putative AMP-binding motifs, the SSGTTGLPKGV and GEICIRG domains | Hibiscus cannabinus |
physiological function | the enzyme is a key regulatory enzyme of the phenylpropanoid pathway that regulates the activation of cinnamic acid, leading to the synthesis of flavonoids and lignin | Hibiscus cannabinus |