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Literature summary for 6.2.1.12 extracted from
- 4-Coumarate:CoA ligase family members from elicitor-treated Sorbus aucuparia cell cultures (2011), J. Plant Physiol., 168, 944-951.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GST-tagged isozyme Sa4CL1 in Escherichia coli | Sorbus aucuparia |
| expression of His6-tagged isozyme Sa4CL2 in Escherichia coli | Sorbus aucuparia |
| expression of His6-tagged isozyme Sa4CL3 in Escherichia coli | Sorbus aucuparia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state MichaelisMenten kinetics | Sorbus aucuparia | |
| 0.0009 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia |  |
| 0.0009 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.0025 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.0054 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.0068 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.0073 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.009 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.0125 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.021 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.023 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.033 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.05 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | isozyme Sa4CL3 contains a 49-amino acid N-terminal extension, which includes a chloroplast sorting signal | Sorbus aucuparia | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can substitute Mg2+ | Sorbus aucuparia | |
| Mg2+ | required | Sorbus aucuparia | |
| Mn2+ | can substitute Mg2+ | Sorbus aucuparia | |
| additional information | strict dependence of Sa4CLs on a divalent cation, no activity with Cu2+ | Sorbus aucuparia |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59500 | - |
x * 59500, about, isozyme Sa4CL1, sequence calculation | Sorbus aucuparia |
| 60100 | - |
x * 60100, about, isozyme Sa4CL2, sequence calculation | Sorbus aucuparia |
| 66200 | - |
x * 66200, about, isozyme Sa4CL3, sequence calculation | Sorbus aucuparia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | Sorbus aucuparia | - |
AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + cinnamate + CoA | Sorbus aucuparia | - |
AMP + diphosphate + cinnamoyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sorbus aucuparia | D5LLN7 | isozyme Sa4CL1; isozyme Sa4CL1 | - |
| Sorbus aucuparia | D5LNB5 | isozyme Sa4CL2; isozyme Sa4CL2 | - |
| Sorbus aucuparia | D5LNB6 | isozyme Sa4CL3; isozyme Sa4CL3 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Sorbus aucuparia | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | - |
Sorbus aucuparia | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + 4-coumarate + CoA | preferred substrate | Sorbus aucuparia | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + caffeate + CoA | - |
Sorbus aucuparia | AMP + diphosphate + caffeoyl-CoA | - |
? | |
| ATP + cinnamate + CoA | - |
Sorbus aucuparia | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
| ATP + ferulate + CoA | - |
Sorbus aucuparia | AMP + diphosphate + ferulyl-CoA | - |
? | |
| additional information | no activity with benzoate and sinapate | Sorbus aucuparia | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59500, about, isozyme Sa4CL1, sequence calculation | Sorbus aucuparia |
| ? | x * 60100, about, isozyme Sa4CL2, sequence calculation | Sorbus aucuparia |
| ? | x * 66200, about, isozyme Sa4CL3, sequence calculation | Sorbus aucuparia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-coumarate:CoA ligase | - |
Sorbus aucuparia |
| Sa4CL1 | - |
Sorbus aucuparia |
| Sa4CL2 | - |
Sorbus aucuparia |
| Sa4CL3 | - |
Sorbus aucuparia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Sorbus aucuparia |
| 37 | - |
- |
Sorbus aucuparia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.3 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.34 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.36 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.37 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.38 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.49 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.58 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.78 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.8 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
| 0.96 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
| 1 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Sorbus aucuparia |
| 7.5 | - |
- |
Sorbus aucuparia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Sorbus aucuparia | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sorbus aucuparia | sequence calculation | - |
5.5 |
| Sorbus aucuparia | sequence calculation | - |
5.6 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogenetically, isozymes Sa4CL1 and Sa4CL2 belong to the class I cluster and Sa4CL3 groups in the class II cluster | Sorbus aucuparia |
| metabolism | the enzyme is involved in the flavonoid biosynthesis from cinnamic acid, it is the last enzyme of the general phenylpropanoid pathway, overview | Sorbus aucuparia |
| physiological function | Sorbus aucuparia cell cultures accumulate biphenyl and dibenzofuran phytoalexins from the starter substrate benzoyl-CoA in response to elicitor treatment involving cinnamate:CoA ligase and benzoate:CoA ligase, but not 4-coumarate:CoA ligase | Sorbus aucuparia |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
| 10 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
| 60 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
| 90 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
| 120 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
| 120 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
| 130 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
| 230 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
| 360 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
| 410 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
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