Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)/pET28a, subcloning in Escherichia coli strain M15 | Pueraria montana var. lobata |
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)/pQE30, subcloning in Escherichia coli strain M15 | Pueraria montana var. lobata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
4-coumarate | pH 7.8, 22°C | Pueraria montana var. lobata | |
0.0091 | - |
4-coumarate | pH 7.8, 22°C | Pueraria montana var. lobata | |
0.0149 | - |
caffeate | pH 7.8, 22°C | Pueraria montana var. lobata | |
0.0884 | - |
ferulate | pH 7.8, 22°C | Pueraria montana var. lobata | |
0.141 | - |
trans-cinnamate | pH 7.8, 22°C | Pueraria montana var. lobata | |
0.664 | - |
trans-cinnamate | pH 7.8, 22°C | Pueraria montana var. lobata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Pueraria montana var. lobata | - |
AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | Pueraria montana var. lobata | - |
AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + ferulate + CoA | Pueraria montana var. lobata | - |
AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + trans-cinnamate + CoA | Pueraria montana var. lobata | - |
AMP + diphosphate + trans-cinnamoyl-CoA | - |
? | |
additional information | Pueraria montana var. lobata | isozyme Pl4CL2 has a broader substrate specificity than Pl4CL1 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pueraria montana var. lobata | U3MJP5 | isozyme Pl4CL2 | - |
Pueraria montana var. lobata | U3MNX0 | isozyme Pl4CL1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no N-terminal extension of Pl4CL2 | Pueraria montana var. lobata | - |
additional information | Pl4CL1 has an N-terminal extension of twenty-one amino acid residues | Pueraria montana var. lobata | - |
root | - |
Pueraria montana var. lobata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Pueraria montana var. lobata | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Pueraria montana var. lobata | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + ferulate + CoA | - |
Pueraria montana var. lobata | AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + trans-cinnamate + CoA | - |
Pueraria montana var. lobata | AMP + diphosphate + trans-cinnamoyl-CoA | - |
? | |
additional information | isozyme Pl4CL2 has a broader substrate specificity than Pl4CL1 | Pueraria montana var. lobata | ? | - |
? | |
additional information | no activity with sinapate | Pueraria montana var. lobata | ? | - |
? | |
additional information | no activity with sinapate, ferulate and caffeate | Pueraria montana var. lobata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-coumarate: coenzyme A ligase | - |
Pueraria montana var. lobata |
Pl4CL1 | - |
Pueraria montana var. lobata |
Pl4CL2 | - |
Pueraria montana var. lobata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Pueraria montana var. lobata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
trans-cinnamate | pH 7.8, 22°C | Pueraria montana var. lobata | |
0.57 | - |
ferulate | pH 7.8, 22°C | Pueraria montana var. lobata | |
0.92 | - |
caffeate | pH 7.8, 22°C | Pueraria montana var. lobata | |
0.97 | - |
trans-cinnamate | pH 7.8, 22°C | Pueraria montana var. lobata | |
1.02 | - |
4-coumarate | pH 7.8, 22°C | Pueraria montana var. lobata | |
1.28 | - |
4-coumarate | pH 7.8, 22°C | Pueraria montana var. lobata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Pueraria montana var. lobata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pueraria montana var. lobata |
Organism | Comment | Expression |
---|---|---|
Pueraria montana var. lobata | expression of Pl4CL2 is not significantly influenced in methyl jasmonate-treated samples | additional information |
Pueraria montana var. lobata | expression of Pl4CL1 is increased more than 3fold in the 24 h methyl jasmonate-treated samples | up |
General Information | Comment | Organism |
---|---|---|
evolution | isozyme Pl4CL1 belongs to the class II 4-coumarate-CoA ligases | Pueraria montana var. lobata |
evolution | isozyme Pl4CL2 belongs to the class I 4-coumarate-CoA ligases | Pueraria montana var. lobata |
physiological function | isozyme Pl4CL1 might play a role in isoflavone biosynthesis | Pueraria montana var. lobata |
physiological function | isozyme Pl4CL2 might function as a housekeeping enzyme concerning lignification | Pueraria montana var. lobata |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.46 | - |
trans-cinnamate | pH 7.8, 22°C | Pueraria montana var. lobata | |
3.3 | - |
trans-cinnamate | pH 7.8, 22°C | Pueraria montana var. lobata | |
6.5 | - |
ferulate | pH 7.8, 22°C | Pueraria montana var. lobata | |
62 | - |
caffeate | pH 7.8, 22°C | Pueraria montana var. lobata | |
112 | - |
4-coumarate | pH 7.8, 22°C | Pueraria montana var. lobata | |
364 | - |
4-coumarate | pH 7.8, 22°C | Pueraria montana var. lobata |