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Literature summary for 6.2.1.12 extracted from

  • Schneider, K.; Hovel, K.; Witzel, K.; Hamberger, B.; Schomburg, D.; Kombrink, E.; Stuible, H.P.
    The substrate specificity-determining amino acid code of 4-coumarate:CoA ligase (2003), Proc. Natl. Acad. Sci. USA, 100, 8601-8606.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
-
 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
L356A only little effect on activity Arabidopsis thaliana
M293P/K320L effectively uses ferulate as substrate, changes in Km for all substrates Arabidopsis thaliana
additional information domain exchange experiments with gramicidine synthetase from Bacillus brevis Arabidopsis thaliana
N256A/M293P/K320L effectively uses ferulate and cinnamate as substrates, 10fold reduced activity with caffeate Arabidopsis thaliana
V355A strongly reduced activity Arabidopsis thaliana
V355A/L356A strongly reduced activity Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 caffeate wild-type enzyme Arabidopsis thaliana
0.022
-
 4-coumarate M293P/K320L mutant enzyme Arabidopsis thaliana
0.03
-
 ferulate M293P/K320L mutant enzyme Arabidopsis thaliana
0.041
-
 caffeate M293P/K320L mutant enzyme Arabidopsis thaliana
0.233
-
 4-coumarate wild-type enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Arabidopsis thaliana plant phenylpropanoid metabolism AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 isoform 4CL2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA plant phenylpropanoid metabolism Arabidopsis thaliana AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + caffeate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + caffeoyl-CoA
-
 ?
ATP + ferulate + CoA very poor substrate for the wild type enzyme, effective substrate for M293P/K320L mutant enzyme Arabidopsis thaliana AMP + diphosphate + feruloyl-CoA
-
 ?