Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
gel filtration | Picea abies |
63000 | - |
1 * 63000, SDS-PAGE | Picea abies |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picea abies | - |
isoenzymes not detected | - |
Purification (Comment) | Organism |
---|---|
- |
Picea abies |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cambium | sap | Picea abies | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Picea abies |
Storage Stability | Organism |
---|---|
-20°C, 10 mM sodium/potassium phosphate, pH 7.0, 42 mM mercaptoethanol, 20% ethyleneglycol, stable for several months | Picea abies |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | 94% of the activity relative to ferulate | Picea abies | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 5-hydroxyferulate + CoA | 18% of the activity relative to ferulate | Picea abies | AMP + diphosphate + 5-hydroxyferuloyl-CoA | - |
? | |
ATP + caffeate + CoA | 86% of the activity relative to ferulate | Picea abies | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + cinnamate + CoA | 14% of the activity relative to ferulate | Picea abies | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
ATP + ferulate + CoA | - |
Picea abies | AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + sinapoate + CoA | no activity | Picea abies | AMP + diphosphate + sinapoyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 63000, SDS-PAGE | Picea abies |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Picea abies |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.5 | 6.8: about 80% of maximal activity, 7.5: maximal activity | Picea abies |