Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | substrate inhibition | Forsythia suspensa | |
ferulate | strong substrate inhibition above 0.2 mM | Forsythia suspensa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
CoA | - |
Forsythia suspensa | |
0.02 | - |
ferulate | - |
Forsythia suspensa | |
0.036 | - |
ATP | - |
Forsythia suspensa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Forsythia suspensa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Forsythia suspensa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Forsythia suspensa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Forsythia suspensa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | young, lignifying | Forsythia suspensa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Forsythia suspensa |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol | Forsythia suspensa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Forsythia suspensa | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + ferulate + CoA | - |
Forsythia suspensa | AMP + diphosphate + feruloyl-CoA | - |
? | |
CTP + ferulate + CoA | 7.7% of the activity relative to ATP | Forsythia suspensa | CMP + diphosphate + feruloyl-CoA | - |
? | |
GTP + ferulate + CoA | 5.7% of the activity relative to ATP | Forsythia suspensa | GMP + diphosphate + feruloyl-CoA | - |
? | |
ITP + ferulate + CoA | 20.2% of the activity relative to ATP | Forsythia suspensa | IMP + diphosphate + feruloyl-CoA | - |
? | |
additional information | in ATP-diphosphate exchange reaction absolute specificity for cinnamate | Forsythia suspensa | ? | - |
? | |
UTP + ferulate + CoA | 12.5% of the activity relative to GTP | Forsythia suspensa | UMP + diphosphate + feruloyl-CoA | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
rapid inactivation | Forsythia suspensa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
ATP-diphosphate exchange reaction | Forsythia suspensa |
7.3 | - |
formation of feruloyl-CoA | Forsythia suspensa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | stable | Forsythia suspensa |