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Literature summary for 6.2.1.12 extracted from

  • Meng, H.; Campbell, W.H.
    Facile enzymic synthesis of caffeoyl CoA (1997), Phytochemistry, 44, 605-608.
No PubMed abstract available

Application

Application Comment Organism
synthesis crude aspen CoA ligase can be effectively and economically used to synthesize caffeoyl CoA Populus sp.

Organism

Organism UniProt Comment Textmining
Populus sp.
-
aspen
-

Source Tissue

Source Tissue Comment Organism Textmining
xylem
-
Populus sp.
-

Storage Stability

Storage Stability Organism
-20°C, stable for 2 or 3 months, crude enzyme extract Populus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA
-
Populus sp. AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 5-hydroxyferulate + CoA 41% of the activity relative to p-hydroxycinnamate Populus sp. AMP + diphosphate + 5-hydroxyferuloyl-CoA
-
?
ATP + caffeate + CoA 61% of the activity relative to p-hydroxycinnamate Populus sp. AMP + diphosphate + caffeoyl-CoA
-
?
ATP + ferulate + CoA 67% of the activity relative to p-hydroxycinnamate Populus sp. AMP + diphosphate + feruloyl-CoA
-
?
ATP + sinapoate + CoA no activity Populus sp. AMP + diphosphate + sinapoyl-CoA
-
?