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Literature summary for 6.2.1.1 extracted from

  • Reger, A.S.; Carney, J.M.; Gulick, A.M.
    Biochemical and crystallographic analysis of substrate binding and conformational changes in acetyl-CoA synthetase (2007), Biochemistry, 46, 6536-6546.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method, crystallographic structures of wild-type enzyme and mutant enzymes R194A, R584A, R584E, K609A, and V386A Salmonella enterica

Protein Variants

Protein Variants Comment Organism
A357V kcat/Km for ATP is 1.2fold higher than wild-type value, kcat/Km for CoA is 3.2fold lower than wild-type value Salmonella enterica
D517G kcat/Km for ATP is 6.5fold lower than wild-type value, kcat/Km for CoA is 9.5fold lower than wild-type value Salmonella enterica
D517P kcat/Km for ATP is 1.4fold lower than wild-type value, kcat/Km for CoA is 23.7fold lower than wild-type value Salmonella enterica
G524L inactive mutant enzyme Salmonella enterica
G524L mutant enzyme is unable to catalyze the complete reaction yet catalyzes the adenylation half-reaction with activity comparable to the wild-type enzyme Salmonella enterica
G524S kcat/Km for ATP is 1.6fold lower than wild-type value, kcat/Km for CoA is 19fold lower than wild-type value Salmonella enterica
K609A inactive mutant enzyme Salmonella enterica
K609A mutation results in an enzyme that is unable to catalyze the adenylate reaction Salmonella enterica
R194A kcat/Km for ATP is 1.1fold higher than wild-type value, kcat/Km for CoA is 6.3fold lower than wild-type value Salmonella enterica
R194E kcat/Km for ATP is 1.2fold lower than wild-type value, kcat/Km for CoA is 4.75fold lower than wild-type value Salmonella enterica
R526A kcat/Km for ATP is 1.2fold higher than wild-type value, kcat/Km for CoA is 9.5fold lower than wild-type value Salmonella enterica
R584A kcat/Km for ATP is 1.2 fold than wild-type value, kcat/Km for CoA is 19fold lower than wild-type value Salmonella enterica
R584E kcat/Km for ATP is 1.1fold higher than wild-type value, kcat/Km for CoA is 21fold lower than wild-type value Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0224
-
ATP pH 7.5, mutant enzyme R526A Salmonella enterica
0.0238
-
ATP pH 7.5, mutant enzyme A357V Salmonella enterica
0.0265
-
ATP pH 7.5, mutant enzyme R584E Salmonella enterica
0.0287
-
ATP pH 7.5, mutant enzyme R194A Salmonella enterica
0.0373
-
ATP pH 7.5, mutant enzyme R194E Salmonella enterica
0.0381
-
ATP pH 7.5, mutant enzyme R584A Salmonella enterica
0.044
-
ATP pH 7.5, mutant enzyme D517P Salmonella enterica
0.05
-
CoA pH 7.5, wild-type enzyme Salmonella enterica
0.0638
-
ATP pH 7.5, mutant enzyme G524S Salmonella enterica
0.0771
-
ATP pH 7.5, wild-type enzyme Salmonella enterica
0.1072
-
CoA pH 7.5, mutant enzyme R194E Salmonella enterica
0.133
-
CoA pH 7.5, mutant enzyme A357V Salmonella enterica
0.1417
-
CoA pH 7.5, mutant enzyme R194A Salmonella enterica
0.205
-
CoA pH 7.5, mutant enzyme R526A Salmonella enterica
0.228
-
CoA pH 7.5, mutant enzyme D517G Salmonella enterica
0.243
-
ATP pH 7.5, mutant enzyme D517G Salmonella enterica
0.3583
-
CoA pH 7.5, mutant enzyme R584A Salmonella enterica
0.426
-
CoA pH 7.5, mutant enzyme R584E Salmonella enterica
0.448
-
CoA pH 7.5, mutant enzyme G524S Salmonella enterica
0.527
-
CoA pH 7.5, mutant enzyme D517P Salmonella enterica

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA
-
Salmonella enterica AMP + diphosphate + acetyl-CoA
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
ATP pH 7.5, wild-type enzyme Salmonella enterica
3.2
-
CoA pH 7.5, wild-type enzyme Salmonella enterica
35
-
CoA pH 7.5, mutant enzyme A357V Salmonella enterica
36.4
-
ATP pH 7.5, mutant enzyme R584E Salmonella enterica
37.4
-
ATP pH 7.5, mutant enzyme R526A Salmonella enterica
38.9
-
ATP pH 7.5, mutant enzyme A357V Salmonella enterica
39.9
-
ATP pH 7.5, mutant enzyme R194A Salmonella enterica
40
-
CoA pH 7.5, mutant enzyme R194E Salmonella enterica
40.5
-
CoA pH 7.5, mutant enzyme R526A Salmonella enterica
40.8
-
CoA pH 7.5, mutant enzyme R584E Salmonella enterica
40.9
-
ATP pH 7.5, mutant enzyme D517P Salmonella enterica
41.1
-
CoA pH 7.5, mutant enzyme R194A Salmonella enterica
41.5
-
CoA pH 7.5, mutant enzyme R584A Salmonella enterica
41.7
-
ATP pH 7.5, mutant enzyme R194E Salmonella enterica
42.4
-
CoA pH 7.5, mutant enzyme D517G Salmonella enterica
42.8
-
ATP pH 7.5, mutant enzyme D517G Salmonella enterica
43.1
-
CoA pH 7.5, mutant enzyme G524S Salmonella enterica
43.8
-
CoA pH 7.5, mutant enzyme D517P Salmonella enterica
46.7
-
ATP pH 7.5, mutant enzyme R584A Salmonella enterica
49.8
-
ATP pH 7.5, mutant enzyme G524S Salmonella enterica
95.1
-
CoA pH 7.5, wild-type enzyme Salmonella enterica
100.6
-
ATP pH 7.5, wild-type enzyme Salmonella enterica