Crystallization (Comment) | Organism |
---|---|
vapor diffusion method, crystallographic structures of wild-type enzyme and mutant enzymes R194A, R584A, R584E, K609A, and V386A | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
A357V | kcat/Km for ATP is 1.2fold higher than wild-type value, kcat/Km for CoA is 3.2fold lower than wild-type value | Salmonella enterica |
D517G | kcat/Km for ATP is 6.5fold lower than wild-type value, kcat/Km for CoA is 9.5fold lower than wild-type value | Salmonella enterica |
D517P | kcat/Km for ATP is 1.4fold lower than wild-type value, kcat/Km for CoA is 23.7fold lower than wild-type value | Salmonella enterica |
G524L | inactive mutant enzyme | Salmonella enterica |
G524L | mutant enzyme is unable to catalyze the complete reaction yet catalyzes the adenylation half-reaction with activity comparable to the wild-type enzyme | Salmonella enterica |
G524S | kcat/Km for ATP is 1.6fold lower than wild-type value, kcat/Km for CoA is 19fold lower than wild-type value | Salmonella enterica |
K609A | inactive mutant enzyme | Salmonella enterica |
K609A | mutation results in an enzyme that is unable to catalyze the adenylate reaction | Salmonella enterica |
R194A | kcat/Km for ATP is 1.1fold higher than wild-type value, kcat/Km for CoA is 6.3fold lower than wild-type value | Salmonella enterica |
R194E | kcat/Km for ATP is 1.2fold lower than wild-type value, kcat/Km for CoA is 4.75fold lower than wild-type value | Salmonella enterica |
R526A | kcat/Km for ATP is 1.2fold higher than wild-type value, kcat/Km for CoA is 9.5fold lower than wild-type value | Salmonella enterica |
R584A | kcat/Km for ATP is 1.2 fold than wild-type value, kcat/Km for CoA is 19fold lower than wild-type value | Salmonella enterica |
R584E | kcat/Km for ATP is 1.1fold higher than wild-type value, kcat/Km for CoA is 21fold lower than wild-type value | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0224 | - |
ATP | pH 7.5, mutant enzyme R526A | Salmonella enterica | |
0.0238 | - |
ATP | pH 7.5, mutant enzyme A357V | Salmonella enterica | |
0.0265 | - |
ATP | pH 7.5, mutant enzyme R584E | Salmonella enterica | |
0.0287 | - |
ATP | pH 7.5, mutant enzyme R194A | Salmonella enterica | |
0.0373 | - |
ATP | pH 7.5, mutant enzyme R194E | Salmonella enterica | |
0.0381 | - |
ATP | pH 7.5, mutant enzyme R584A | Salmonella enterica | |
0.044 | - |
ATP | pH 7.5, mutant enzyme D517P | Salmonella enterica | |
0.05 | - |
CoA | pH 7.5, wild-type enzyme | Salmonella enterica | |
0.0638 | - |
ATP | pH 7.5, mutant enzyme G524S | Salmonella enterica | |
0.0771 | - |
ATP | pH 7.5, wild-type enzyme | Salmonella enterica | |
0.1072 | - |
CoA | pH 7.5, mutant enzyme R194E | Salmonella enterica | |
0.133 | - |
CoA | pH 7.5, mutant enzyme A357V | Salmonella enterica | |
0.1417 | - |
CoA | pH 7.5, mutant enzyme R194A | Salmonella enterica | |
0.205 | - |
CoA | pH 7.5, mutant enzyme R526A | Salmonella enterica | |
0.228 | - |
CoA | pH 7.5, mutant enzyme D517G | Salmonella enterica | |
0.243 | - |
ATP | pH 7.5, mutant enzyme D517G | Salmonella enterica | |
0.3583 | - |
CoA | pH 7.5, mutant enzyme R584A | Salmonella enterica | |
0.426 | - |
CoA | pH 7.5, mutant enzyme R584E | Salmonella enterica | |
0.448 | - |
CoA | pH 7.5, mutant enzyme G524S | Salmonella enterica | |
0.527 | - |
CoA | pH 7.5, mutant enzyme D517P | Salmonella enterica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | - |
Salmonella enterica | AMP + diphosphate + acetyl-CoA | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
ATP | pH 7.5, wild-type enzyme | Salmonella enterica | |
3.2 | - |
CoA | pH 7.5, wild-type enzyme | Salmonella enterica | |
35 | - |
CoA | pH 7.5, mutant enzyme A357V | Salmonella enterica | |
36.4 | - |
ATP | pH 7.5, mutant enzyme R584E | Salmonella enterica | |
37.4 | - |
ATP | pH 7.5, mutant enzyme R526A | Salmonella enterica | |
38.9 | - |
ATP | pH 7.5, mutant enzyme A357V | Salmonella enterica | |
39.9 | - |
ATP | pH 7.5, mutant enzyme R194A | Salmonella enterica | |
40 | - |
CoA | pH 7.5, mutant enzyme R194E | Salmonella enterica | |
40.5 | - |
CoA | pH 7.5, mutant enzyme R526A | Salmonella enterica | |
40.8 | - |
CoA | pH 7.5, mutant enzyme R584E | Salmonella enterica | |
40.9 | - |
ATP | pH 7.5, mutant enzyme D517P | Salmonella enterica | |
41.1 | - |
CoA | pH 7.5, mutant enzyme R194A | Salmonella enterica | |
41.5 | - |
CoA | pH 7.5, mutant enzyme R584A | Salmonella enterica | |
41.7 | - |
ATP | pH 7.5, mutant enzyme R194E | Salmonella enterica | |
42.4 | - |
CoA | pH 7.5, mutant enzyme D517G | Salmonella enterica | |
42.8 | - |
ATP | pH 7.5, mutant enzyme D517G | Salmonella enterica | |
43.1 | - |
CoA | pH 7.5, mutant enzyme G524S | Salmonella enterica | |
43.8 | - |
CoA | pH 7.5, mutant enzyme D517P | Salmonella enterica | |
46.7 | - |
ATP | pH 7.5, mutant enzyme R584A | Salmonella enterica | |
49.8 | - |
ATP | pH 7.5, mutant enzyme G524S | Salmonella enterica | |
95.1 | - |
CoA | pH 7.5, wild-type enzyme | Salmonella enterica | |
100.6 | - |
ATP | pH 7.5, wild-type enzyme | Salmonella enterica |