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Literature summary for 6.1.1.9 extracted from

  • Olmedo-Verd, E.; Santamaria-Gomez, J.; Ochoa de Alda, J.A.; Ribas de Pouplana, L.; Luque, I.
    Membrane anchoring of aminoacyl-tRNA synthetases by convergent acquisition of a novel protein domain (2011), J. Biol. Chem., 286, 41057-41068.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Anabaena sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00061
-
 tRNAVal(GAC) in 100 mM HEPES (pH 7.2), 20 mM KCl, 30 mM MgCl2, at 30°C Anabaena sp.
0.00108
-
 tRNAVal(UAC-2) in 100 mM HEPES (pH 7.2), 20 mM KCl, 30 mM MgCl2, at 30°C Anabaena sp.
0.00208
-
 tRNAVal(UAC) in 100 mM HEPES (pH 7.2), 20 mM KCl, 30 mM MgCl2, at 30°C Anabaena sp.
0.1926
-
 L-valine in 100 mM HEPES (pH 7.2), 20 mM KCl, 30 mM MgCl2, at 30°C Anabaena sp.
0.2005
-
 ATP in 100 mM HEPES (pH 7.2), 20 mM KCl, 30 mM MgCl2, at 30°C Anabaena sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
thylakoid
-
 Anabaena sp. 9579
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-valine + tRNAVal Anabaena sp.
-
 AMP + diphosphate + L-valyl-tRNAVal
-
 ?
ATP + L-valine + tRNAVal Anabaena sp. PCC 7120
-
 AMP + diphosphate + L-valyl-tRNAVal
-
 ?

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
-
-
Anabaena sp. PCC 7120
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography, and PD-10 gel filtration Anabaena sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-valine + tRNAVal
-
 Anabaena sp. AMP + diphosphate + L-valyl-tRNAVal
-
 ?
ATP + L-valine + tRNAVal
-
 Anabaena sp. PCC 7120 AMP + diphosphate + L-valyl-tRNAVal
-
 ?
ATP + L-valine + tRNAVal(GAC)
-
 Anabaena sp. AMP + diphosphate + L-valyl-tRNAVal(GAC)
-
 ?
ATP + L-valine + tRNAVal(GAC)
-
 Anabaena sp. PCC 7120 AMP + diphosphate + L-valyl-tRNAVal(GAC)
-
 ?
ATP + L-valine + tRNAVal(UAC)
-
 Anabaena sp. AMP + diphosphate + L-valyl-tRNAVal(UAC)
-
 ?
ATP + L-valine + tRNAVal(UAC)
-
 Anabaena sp. PCC 7120 AMP + diphosphate + L-valyl-tRNAVal(UAC)
-
 ?
ATP + L-valine + tRNAVal(UAC-2)
-
 Anabaena sp. AMP + diphosphate + L-valyl-tRNAVal(UAC-2)
-
 ?
ATP + L-valine + tRNAVal(UAC-2)
-
 Anabaena sp. PCC 7120 AMP + diphosphate + L-valyl-tRNAVal(UAC-2)
-
 ?

Synonyms

Synonyms Comment Organism
ValRS
-
 Anabaena sp.
Valyl-tRNA synthetase
-
 Anabaena sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.074
-
 L-valine at pH and °C Anabaena sp.

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Anabaena sp.