Literature summary for 6.1.1.9 extracted from

Fukunaga, R.; Yokoyama, S.
Structural basis for non-cognate amino acid discrimination by the valyl-tRNA synthetase editing domain (2005), J. Biol. Chem., 280, 29937-29945.

Search for more literature for 6.1.1.9

Crystallization (Commentary)

Crystallization (Comment)	Organism
1.7 A resolution crystal structure of the ValRS editing domain and 1.7 A resolution crystal structure of the editing domain bound with [N-(L-threonyl)-sulfamoyl]adenosine, hanging drop vapor diffusion method	Thermus thermophilus

Protein Variants

Protein Variants	Comment	Organism
D276A	valylation activity is similar to that of wild-type enzyme	Thermus thermophilus
D279A	valylation activity is similar to that of wild-type enzyme, efficiently produces Thr-tRNAVal, drastic decrease in ATP consumption rate, severe deficiency in deacylation activity with Thr-tRNAVal compared to wild-type enzyme	Thermus thermophilus
F264A	valylation activity is similar to that of wild-type enzyme, about 15% decrease in ATP consumption rate compared to wild-type enzyme, somewhat reduced deacylation activityith Thr-tRNAVal, mutation impairs editing activity of ValRS	Thermus thermophilus
K270A	slight reduction of valylation activity, efficiently produces Thr-tRNAVal, drastic decrease in ATP consumption rate, severe deficiency in deacylation activity with Thr-tRNAVal compared to wild-type enzyme	Thermus thermophilus
R216A	valylation activity is similar to that of wild-type enzyme, about 15% decrease in ATP consumption rate compared to wild-type enzyme, somewhat reduced deacylation activity with Thr-tRNAVal	Thermus thermophilus
T272A	valylation activity is similar to that of wild-type enzyme, about 15% decrease in ATP consumption rate compared to wild-type enzyme, somewhat reduced deacylation activity with Thr-tRNAVal	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	P96142	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-valine + tRNAVal	-	Thermus thermophilus	AMP + diphosphate + L-valyl-tRNAVal	-	?

Synonyms

Synonyms	Comment	Organism
ValRS	-	Thermus thermophilus

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Release 2023.1 (January 2023)