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Literature summary for 6.1.1.9 extracted from

  • Bergmann, F.H.; Berg, P.; Dieckmann, M.
    The Enzymic synthesis of amino acyl derivatives of ribonucleic acid. II. The preparation of leucyl-, valyl-, isoleucyl-, and methionyl ribonucleic acid synthetases from Escherichia coli (1961), J. Biol. Chem., 236, 1735-1740.
No PubMed abstract available
Search for more literature for 6.1.1.9

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates Escherichia coli

General Stability

General Stability Organism
relatively unstable in the absence of a sulfhydryl-containing compound Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
D-allo-2-Amino-3-chlorobutyrate
-
 Escherichia coli
DL-2-amino-3-chlorobutyrate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 valine
-
 Escherichia coli
0.33
-
 DL-2-amino-3-chlorobutyrate
-
 Escherichia coli
1
-
 DL-allo-2-amino-3-chlorobutyrate
-
 Escherichia coli
3.7
-
 DL-2-aminobutyrate
-
 Escherichia coli
12
-
 DL-threonine
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + DL-2-amino-3-chlorobutyrate + tRNAVal as effective as valine Escherichia coli AMP + diphosphate + DL-2-amino-3-chlorobutyryl-tRNAVal
-
 ?
ATP + DL-2-aminobutyrate + tRNAVal 30% of the activity with valine Escherichia coli AMP + diphosphate + DL-2-aminobutyryl-tRNAVal
-
 ?
ATP + DL-allo-2-amino-3-chlorobutyrate + tRNAVal 15% of the activity with valine Escherichia coli AMP + diphosphate + DL-allo-2-amino-3-chlorobutyryl-tRNAVal
-
 ?
ATP + DL-threonine + tRNAVal
-
 Escherichia coli AMP + diphosphate + DL-threonyl-tRNAVal
-
 ?
ATP + L-threonine + tRNAVal 28% of the activity with valine Escherichia coli AMP + diphosphate + L-threonyl-tRNAVal
-
 ?
ATP + L-valine + tRNAVal
-
 Escherichia coli AMP + diphosphate + L-valyl-tRNAVal
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 in Tris glycine or glycine buffer Escherichia coli