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Literature summary for 6.1.1.7 extracted from

  • Wu, M.X.; Filley, S.J.; Xiong, J.; Lee, J.J.; Hill, K.A.W.
    A cysteine in the C-terminal region of alanyl-tRNA synthetase is important for aminoacylation activity (1994), Biochemistry, 33, 12260-12266.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C290S mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency Escherichia coli
C665S mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency Escherichia coli
C76S mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Escherichia coli
monobromobimane
-
Escherichia coli
p-Hydroxymercuriphenylsulfonic acid
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
ATP wild-type enzyme Escherichia coli
0.095
-
ATP Cys665Ser mtant Escherichia coli
0.12
-
ATP Cys412Ser mutant Escherichia coli
0.18
-
ATP Cys76Ser mutant Escherichia coli
0.21
-
ATP Cys290Ser mutant Escherichia coli
0.23
-
alanine Cys665Ser mutant Escherichia coli
0.33
-
alanine mutant Cys76Ser Escherichia coli
0.34
-
alanine wild-type Escherichia coli
0.47
-
alanine Cys412Ser mutant Escherichia coli
0.5
-
alanine Cys290Ser mutant Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc thightly bound Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
wild-type and mutant enzymes with replacement ofcysteine residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-alanine + tRNAAla
-
Escherichia coli AMP + diphosphate + L-alanyl-tRNAAla
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
79
-
ATP Cys412Ser mutant Escherichia coli
84
-
ATP wild-type Escherichia coli
89
-
ATP Cys665Ser mutant Escherichia coli
110
-
ATP Cys290Ser mutant Escherichia coli
120
-
ATP Cys76Ser mutant Escherichia coli