Protein Variants | Comment | Organism |
---|---|---|
C290S | mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency | Escherichia coli |
C665S | mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency | Escherichia coli |
C76S | mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli | |
monobromobimane | - |
Escherichia coli | |
p-Hydroxymercuriphenylsulfonic acid | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
ATP | wild-type enzyme | Escherichia coli | |
0.095 | - |
ATP | Cys665Ser mtant | Escherichia coli | |
0.12 | - |
ATP | Cys412Ser mutant | Escherichia coli | |
0.18 | - |
ATP | Cys76Ser mutant | Escherichia coli | |
0.21 | - |
ATP | Cys290Ser mutant | Escherichia coli | |
0.23 | - |
alanine | Cys665Ser mutant | Escherichia coli | |
0.33 | - |
alanine | mutant Cys76Ser | Escherichia coli | |
0.34 | - |
alanine | wild-type | Escherichia coli | |
0.47 | - |
alanine | Cys412Ser mutant | Escherichia coli | |
0.5 | - |
alanine | Cys290Ser mutant | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | thightly bound | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and mutant enzymes with replacement ofcysteine residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAAla | - |
Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
79 | - |
ATP | Cys412Ser mutant | Escherichia coli | |
84 | - |
ATP | wild-type | Escherichia coli | |
89 | - |
ATP | Cys665Ser mutant | Escherichia coli | |
110 | - |
ATP | Cys290Ser mutant | Escherichia coli | |
120 | - |
ATP | Cys76Ser mutant | Escherichia coli |