Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L73A | mutant enzymes with replacement of Lys73 with Gln, Asn, Ala or Glu show reduction in catalytic efficiency in aminoacylation assay. Glu substitution causes a 5-fold decrease in affinity for alanine | Escherichia coli |
L73E | mutant enzymes with replacement of Lys73 with Gln, Asn, Ala or Glu show reduction in catalytic efficiency in aminoacylation assay. Glu substitution causes a 5-fold decrease in affinity for alanine | Escherichia coli |
L73N | mutant enzymes with replacement of Lys73 with Gln, Asn, Ala or Glu show reduction in catalytic efficiency in aminoacylation assay. Glu substitution causes a 5-fold decrease in affinity for alanine | Escherichia coli |
L73Q | mutant enzymes with replacement of Lys73 with Gln, Asn, Ala or Glu show reduction in catalytic efficiency in aminoacylation assay. Glu substitution causes a 5-fold decrease in affinity for alanine | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
tRNAAla | wild-type AlaRS, aminoacylation | Escherichia coli | |
0.022 | - |
tRNAAla | mutant AlaRS(K73Q), aminoacylation | Escherichia coli | |
0.023 | - |
tRNA | mutant AlaRS(K73E), aminoacylation | Escherichia coli | |
0.028 | - |
tRNAAla | mutant AlaR(K73A), aminoacylation | Escherichia coli | |
0.14 | - |
ATP | wild-type AlaRS and mutant AlaRS(K73E), AlaRS(K73Q), ATP-diphosphate exchange | Escherichia coli | |
0.2 | - |
ATP | mutant AlaRS(K73A), ATP-diphosphate exchange | Escherichia coli | |
0.3 | - |
alanine | wild-type AlaRS and mutant AlaRS(K73Q), ATP-diphosphate exchange | Escherichia coli | |
0.31 | - |
alanine | mutant AlaRS(K73A), ATP-diphosphate exchange | Escherichia coli | |
1.41 | - |
alanine | mutant AlaRS(K73E), ATP-diphosphate exchange | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and mutant enzymes with replacement of Lys73 with Gln, Asn, Ala or Glu | - |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNA | - |
Escherichia coli | ? | - |
? | |
ATP + L-alanine + tRNAAla | - |
Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
additional information | alanine dependent ATP-diphosphate exchange: alanine + ATP + enzyme /alanine-AMP-enzyme + diphosphate | Escherichia coli | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
tRNAAla | mutant AlaRS(K73E), aminoacylation | Escherichia coli | |
1.4 | - |
tRNAAla | mutant AlaRS(K73A), aminoacylation | Escherichia coli | |
1.5 | - |
tRNAAla | AlaRS, aminoacylation | Escherichia coli | |
1.7 | - |
tRNAAla | mutant AlaRS(K73Q), ATP-diphosphate exchange | Escherichia coli | |
82 | - |
ATP | mutant AlaRS(K73Q), ATP-diphosphate exchange | Escherichia coli | |
89 | - |
ATP | AlaRS, ATP-diphosphate exchange | Escherichia coli | |
120 | - |
ATP | AlaRS(K73A), ATP-diphosphate exchange | Escherichia coli |