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Literature summary for 6.1.1.6 extracted from

  • Takita, T.; Nakagoshi, M.; Inouye, K.; Tonomura, B.i.
    Lysyl-tRNA synthetase from Bacillus stearothermophilus: The Trp314 residue is shielded in a non-polar environment and is responsible for the fluorescence changes observed in the amino acid activation reaction (2003), J. Mol. Biol., 325, 677-695.
    View publication on PubMed
Search for more literature for 6.1.1.6

Protein Variants

Protein Variants Comment Organism
W314F site-directed mutagenesis, activity is similar to the wild-type enzyme Geobacillus stearothermophilus
W314f/W332F site-directed mutagenesis, slightly reduced activity Geobacillus stearothermophilus
W332F site-directed mutagenesis, activity is slightly reduced, but the binding of L-lysine is altered, increased Km for L-lysine Geobacillus stearothermophilus
Y271F site-directed mutagenesis, highly increased Km for L-lysine in the ATP-diphosphate exchange reaction Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 L-lysine ATP-diphosphate exchange reaction, recombinant wild-type enzyme and mutant W314F/W332F, pH 8.0, 37°C Geobacillus stearothermophilus
0.01
-
 L-lysine ATP-diphosphate exchange reaction, recombinant mutant W314F, pH 8.0, 37°C Geobacillus stearothermophilus
0.016
-
 L-lysine aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C Geobacillus stearothermophilus
0.017
-
 L-lysine aminoacylation reaction, recombinant mutants W314F and W314F/W332F, pH 8.0, 37°C Geobacillus stearothermophilus
0.018
-
 L-lysine ATP-diphosphate exchange reaction, recombinant mutant W332F, pH 8.0, 37°C Geobacillus stearothermophilus
0.022
-
 L-lysine aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C Geobacillus stearothermophilus
0.038
-
 ATP aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C Geobacillus stearothermophilus
0.043
-
 ATP aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C Geobacillus stearothermophilus
0.049
-
 ATP aminoacylation reaction, recombinant mutant W314F, pH 8.0, 37°C Geobacillus stearothermophilus
0.066
-
 ATP aminoacylation reaction, recombinant mutant W314F/W332F, pH 8.0, 37°C Geobacillus stearothermophilus
8.1
-
 L-lysine ATP-diphosphate exchange reaction, recombinant mutant Y271F, pH 8.0, 37°C Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + lysine + tRNALys Geobacillus stearothermophilus
-
 AMP + L-lysyl-tRNALys + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
 homogenously purified recombinant class II enzyme
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys Trp314 is involved in binding of L-lysine, binding renders the non-polar microenvironment of the residue more polar Geobacillus stearothermophilus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + lysine + tRNALys
-
 Geobacillus stearothermophilus AMP + L-lysyl-tRNALys + diphosphate
-
 ?
ATP + lysine + tRNALys two-step reaction mechansim, the first step of formation of the aminoacylated enzyme-AMP intermediate is reversible, the second of amino acid transfer to the tRNA is not, binding of L-lysine alone influences the fluorescence of the enzyme, while binding of ATP does not Geobacillus stearothermophilus AMP + L-lysyl-tRNALys + diphosphate
-
 ?
additional information the enzyme also performs the ATP-diphosphate exchange reaction Geobacillus stearothermophilus ?
-
 ?

Synonyms

Synonyms Comment Organism
L-Lysine-transfer RNA ligase
-
 Geobacillus stearothermophilus
Lysine translase
-
 Geobacillus stearothermophilus
Lysine--tRNA ligase
-
 Geobacillus stearothermophilus
Lysine-tRNA synthetase
-
 Geobacillus stearothermophilus
LysRS
-
 Geobacillus stearothermophilus
Lysyl-transfer ribonucleate synthetase
-
 Geobacillus stearothermophilus
Lysyl-transfer RNA synthetase
-
 Geobacillus stearothermophilus
Lysyl-tRNA synthetase
-
 Geobacillus stearothermophilus
Synthetase, lysyl-transfer ribonucleate
-
 Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 ATP ATP-diphosphate exchange reaction, recombinant mutant Y271F, pH 8.0, 37°C Geobacillus stearothermophilus
3 6 L-lysine aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C Geobacillus stearothermophilus
3.13
-
 L-lysine aminoacylation reaction, recombinant mutant W314F, pH 8.0, 37°C Geobacillus stearothermophilus
3.13
-
 L-lysine aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C Geobacillus stearothermophilus
3.23
-
 L-lysine aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C Geobacillus stearothermophilus
3.3
-
 L-lysine aminoacylation reaction, recombinant mutant W314F/W332F, pH 8.0, 37°C Geobacillus stearothermophilus
3.53
-
 L-lysine aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C Geobacillus stearothermophilus
3.58
-
 L-lysine aminoacylation reaction, recombinant mutant W314F, pH 8.0, 37°C Geobacillus stearothermophilus
34.7
-
 ATP ATP-diphosphate exchange reaction, recombinant mutant Y271F, pH 8.0, 37°C Geobacillus stearothermophilus
36.4
-
 ATP ATP-diphosphate exchange reaction, recombinant mutant W332F, pH 8.0, 37°C Geobacillus stearothermophilus
37.3
-
 ATP ATP-diphosphate exchange reaction, recombinant mutant W314F/W332F, pH 8.0, 37°C Geobacillus stearothermophilus
38.6
-
 ATP ATP-diphosphate exchange reaction, recombinant mutant W314F, pH 8.0, 37°C Geobacillus stearothermophilus
42.8
-
 ATP ATP-diphosphate exchange reaction, recombinant wild-type enzyme, pH 8.0, 37°C Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Geobacillus stearothermophilus