Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, genomic organization, expression of cytoplasmic isozyme in Escherichia coli strain BL21(DE3) as His-tagged enzymes, expression in human osteosarcoma cell line 143B | Homo sapiens |
DNA sequence determination and analysis, genomic organization, expression of mitochondrial isozyme in Escherichia coli strain BL21(DE3) as His-tagged enzymes, expression in human osteosarcoma cell line 143B | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | cytoplasmic isozyme | Homo sapiens | 5737 | - |
mitochondrion | mitochondrial isozyme | Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | Homo sapiens | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q15046 | 1 gene encodes a cytoplasmic isozyme, the second encodes both a cytoplasmic and mitochondrial isozyme, which are differenciated by alternative splicing | - |
Homo sapiens | Q15046 | 1 gene encodes a cytoplasmic isozyme, the second encodes both a cytoplasmic and mitochondrial isozyme, which are differentiated by alternative splicing | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00002 | - |
purified recombinant isozyme, mitochondrial tRNALys substrate | Homo sapiens |
0.0001 | - |
purified recombinant isozyme, mitochondrial tRNALys substrate | Homo sapiens |
0.0032 | - |
purified recombinant isozyme, cytoplasmic tRNALys substrate | Homo sapiens |
0.0133 | - |
purified recombinant isozyme, cytoplasmic tRNALys substrate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | - |
Homo sapiens | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | isozyme shows different activities with cytoplasmic and mitochondrial tRNALys | Homo sapiens | AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-Lysine-transfer RNA ligase | - |
Homo sapiens |
Lysine translase | - |
Homo sapiens |
Lysine--tRNA ligase | - |
Homo sapiens |
Lysine-tRNA synthetase | - |
Homo sapiens |
LysRS | - |
Homo sapiens |
Lysyl-transfer ribonucleate synthetase | - |
Homo sapiens |
Lysyl-transfer RNA synthetase | - |
Homo sapiens |
Lysyl-tRNA synthetase | - |
Homo sapiens |
Synthetase, lysyl-transfer ribonucleate | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |