Literature summary for 6.1.1.6 extracted from

Takita, T.; Shimizu, N.; Sukata, T.; Hashimoto, S.; Akita, E.; Yokota, T.; Esaki, N.; Soda, K.; Inouye, K.; Tonomura, B.i.
Lysyl-tRNA synthetase of Bacillus stearothermophilus:molecular cloning and expression of the gene (2000), Biosci. Biotechnol. Biochem., 64, 432-437.

Search for more literature for 6.1.1.6

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, expression as T7-tagged enzyme in Escherichia coli BL21(DE3)	Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.016	-	L-lysine	native enzyme, pH 8.0, 30°C	Geobacillus stearothermophilus
0.023	-	ATP	native enzyme, pH 8.0, 30°C	Geobacillus stearothermophilus
0.023	-	L-lysine	recombinant T7-tagged enzyme, pH 8.0, 30°C	Geobacillus stearothermophilus
0.024	-	L-lysine	recombinant enzyme without t7-tag	Geobacillus stearothermophilus
0.041	-	ATP	recombinant T7-tagged enzyme, pH 8.0, 30°C	Geobacillus stearothermophilus
0.043	-	ATP	recombinant enzyme without t7-tag	Geobacillus stearothermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70600	-	recombinant enzyme, gel filtration	Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-lysine + tRNALys	Geobacillus stearothermophilus	-	AMP + L-lysyl-tRNALys + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	Q9RHV9	NCA1503, native purified class II enzyme	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant T7-tagged enzyme from Escherichia coli BL21(DE3)	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-lysine + tRNALys	-	Geobacillus stearothermophilus	AMP + L-lysyl-tRNALys + diphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
L-Lysine-transfer RNA ligase	-	Geobacillus stearothermophilus
Lysine translase	-	Geobacillus stearothermophilus
Lysine--tRNA ligase	-	Geobacillus stearothermophilus
Lysine-tRNA synthetase	-	Geobacillus stearothermophilus
LysRS	-	Geobacillus stearothermophilus
Lysyl-transfer ribonucleate synthetase	-	Geobacillus stearothermophilus
Lysyl-transfer RNA synthetase	-	Geobacillus stearothermophilus
Lysyl-tRNA synthetase	-	Geobacillus stearothermophilus
Synthetase, lysyl-transfer ribonucleate	-	Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.2	-	L-lysine	native enzyme, pH 8.0, 30°C	Geobacillus stearothermophilus
3.6	-	L-lysine	recombinant enzyme without t7-tag	Geobacillus stearothermophilus
4.1	-	L-lysine	recombinant T7-tagged enzyme, pH 8.0, 30°C	Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Geobacillus stearothermophilus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)