Application | Comment | Organism |
---|---|---|
medicine | inhibition of the enzyme constitutes a potential treatment for African sleeping sickness | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
D539A/W541A | catalytically inactive | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-fluoro-9-(5-O-phosphono-alpha-L-arabinofuranosyl)-9H-purin-6-amine | - |
Trypanosoma brucei | |
2-fluoroadenosine | - |
Trypanosoma brucei | |
3'-amino-3'-deoxy-N,N-dimethyladenosine | - |
Trypanosoma brucei | |
4,6-dideoxy-4-[[N-(14-methylpentadecanoyl)glycyl]amino]-N-9H-purin-6-ylhexopyranosylamine | - |
Trypanosoma brucei | |
9-(3-deoxy-alpha-L-threo-pentofuranosyl)-9H-purin-6-amine | - |
Trypanosoma brucei | |
9-(5-O-acetyl-alpha-L-arabinofuranosyl)-9H-purin-6-amine | - |
Trypanosoma brucei | |
9-(alpha-D-arabinofuranosyl)-9H-purin-6-amine | competitive inhibition | Trypanosoma brucei | |
9-(alpha-L-arabinofuranosyl)-9H-purin-6-amine | - |
Trypanosoma brucei | |
9-(alpha-L-arabinofuranosyl)-N-(3-methylbut-2-en-1-yl)-9H-purin-6-amine | - |
Trypanosoma brucei | |
9-(beta-D-arabinofuranosyl)-9H-purin-6-amine | - |
Trypanosoma brucei | |
9-[5-deoxy-5-[(methylsulfonyl)amino]-alpha-L-arabinofuranosyl]-9H-purin-6-amine | - |
Trypanosoma brucei | |
9-[5-O-(dimethoxyphosphoryl)-alpha-L-arabinofuranosyl]-9H-purin-6-amine | - |
Trypanosoma brucei | |
9-[5-O-[tert-butyl(dimethyl)silyl]-alpha-L-arabinofuranosyl]-9H-purin-6-amine | - |
Trypanosoma brucei | |
N8,N8-dimethyl-9-pentofuranosyl-9H-purine-6,8-diamine | - |
Trypanosoma brucei | |
NSC70422 | - |
Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000004 | - |
L-isoleucine | pH and temperature not specified in the publication | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
x * 110000, SDS-PAGE | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | Trypanosoma brucei | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | - |
Trypanosoma brucei | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 110000, SDS-PAGE | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
IleRS | - |
Trypanosoma brucei |
Isoleucyl-tRNA synthetase | - |
Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma brucei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000027 | - |
9-(alpha-D-arabinofuranosyl)-9H-purin-6-amine | pH and temperature not specified in the publication | Trypanosoma brucei | |
0.0000027 | - |
NSC70422 | pH and temperature not specified in the publication | Trypanosoma brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000141 | - |
pH and temperature not specified in the publication | Trypanosoma brucei | 9-(alpha-D-arabinofuranosyl)-9H-purin-6-amine | |
0.0000141 | - |
pH and temperature not specified in the publication | Trypanosoma brucei | NSC70422 |