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Literature summary for 6.1.1.5 extracted from

  • Baouz, S.; Schmitter, J.M.; Chenoune, L.; Beauvallet, C.; Blanquet, S.; Woisard, A.; Hountondji, C.
    Primary structure revision and active site mapping of E. coli isoleucyl-tRNA synthetase by means of maldi mass spectrometry (2009), Open Biochem. J., 3, 26-38.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.5

Inhibitors

Inhibitors Comment Organism Structure
(3S)-3-amino-1-bromo-4-methylhexan-2-one labeling reagent Escherichia coli
(3S)-3-amino-1-bromo-4-methylpentan-2-one labeling reagent Escherichia coli
(3S)-3-amino-1-bromo-4-phenylbutan-2-one labeling reagent Escherichia coli
(3S)-3-amino-1-bromoheptan-2-one labeling reagent Escherichia coli
2',3'-dialdehyde of tRNAile used to label the binding site for the 3'end of tRNA on the synthetase, incubation of the reagent with IleRS results in a rapid loss of tRNAIle aminoacylation and isoleucine-dependent isotopic ATP-PPi exchange activities Escherichia coli
diethyl dicarbonate
-
 Escherichia coli
pyridoxal 5'-diphospho-5'-adenosine affinity labeling reagent for the ATP-binding site, incubation of the reagent with IleRS results in a rapid loss of tRNAIle aminoacylation and isoleucine-dependent isotopic ATP-PPi exchange activities Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-isoleucine + tRNAIle Escherichia coli
-
 AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
ATP + L-isoleucine + tRNAIle Escherichia coli EM20031
-
 AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli EM20031
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-isoleucine + tRNAIle
-
 Escherichia coli AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
ATP + L-isoleucine + tRNAIle
-
 Escherichia coli EM20031 AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?

Synonyms

Synonyms Comment Organism
IleRS
-
 Escherichia coli
Isoleucyl-tRNA synthetase
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli